Anti-Calnexin抗体[CANX/1543]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [CANX/1543] (AB238078)

Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for Calnexin using ab238078 at 2 μg/ml in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-IFNAR1 antibody [EPR6244] (ab124764) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124764 was shown to bind specifically to IFNAR1. A band was observed at 116 kDa in wild-type HeLa cell lysates with no signal observed at this size in IFNAR1 knockout cell line. To generate this image, wild-type and IFNAR1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Interferon alpha/beta receptor 1 antibody [EPR6244] (<a href='/products/primary-antibodies/interferon-alpha-beta-receptor-1-antibody-epr6244-ab124764'>ab124764</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human IFNAR1 (Interferon alpha/beta receptor 1) knockout HeLa cell line (<a href='/products/cell-lines/human-ifnar1-interferon-alpha-beta-receptor-1-knockout-hela-cell-line-ab261782'>ab261782</a>)

Lane 2:

IFNAR1 knockout HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 64 kDa

Observed band size: 116 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-TTF1/Nkx2-1 antibody [EP1584Y] (ab314937) staining at 1/2000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta.

In Western blot, ab314937 was shown to bind specifically to NKX2-1/TTF-1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit.

Secondary antibodies used were HRP conjugated Streptavidin at 0.1 µg/ml and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Biotin Anti-TTF1/Nkx2-1 antibody [EP1584Y] (<a href='/products/primary-antibodies/biotin-ttf1-nkx2-1-antibody-ep1584y-ab314937'>ab314937</a>) at 1/2000 dilution

Lane 1:

Hela cell lysate at 20 µg

Lane 2:

HEK-293 cell lysate at 20 µg

Secondary

Lane 1:

HRP conjugated Streptavidin at 0.1 µg/mL

Lane 2:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 39 kDa,75 kDa

true

Exposure time: 20min

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-NUAK2 antibody [EPR28627-717] ab322265 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 75 kDa in Wild-type A549 cell lysates with no signal observed at this size in NUAK2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-NUAK2 antibody [EPR28627-717] (<a href='/products/primary-antibodies/nuak2-antibody-epr28627-717-ab322265'>ab322265</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

NUAK2 knockout A549 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 70 kDa

Observed band size: 75 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR25194-152] to P2X4 ab303496 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 58, 73 kDa in Wild-type A549 cell lysates with no signal observed at this size in P2RX4 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-P2X4 antibody [EPR25194-152] (<a href='/products/primary-antibodies/p2x4-antibody-epr25194-152-ab303496'>ab303496</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

P2RX4 knockout A549 at 20 µg

Lane 3:

Wild-type HEK-293T BOILED at 20 µg

Lane 4:

P2RX4 knockout HEK-293T BOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 58 kDa,73 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab254222 was shown to bind specifically to Angiotensin Converting Enzyme 1. A band was observed at 200 kDa in wild-type SKNFI cell lysates with no signal observed at this size in Ace knockout cell line ab288707. To generate this image, wild-type and Ace knockout SKNFI cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

Lanes 1 - 4:

Western blot - Anti-Angiotensin Converting Enzyme 1 antibody [EPR22291-247] (<a href='/products/primary-antibodies/angiotensin-converting-enzyme-1-antibody-epr22291-247-ab254222'>ab254222</a>) at 1/1000 dilution

Lane 1:

Wild-type SKNFI cell lysate at 20 µg

Lane 2:

Ace knockout SKNFI cell lysate at 20 µg

Lane 3:

Human Lung cell lysate at 20 µg

Lane 4:

HUVEC cell lysate at 20 µg

Predicted band size: 150 kDa

Observed band size: 200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-RET antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 150 kDa in Wild-type MCF7 UNBOILED cell lysates with no signal observed at this size in RET knockout MCF7 UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Ret (E1N8X) XP Rabbit mAb at 1/1000 dilution

Lane 1:

Wild-type MCF7 UNBOILED at 20 µg

Lane 2:

Western blot - Human RET knockout MCF7 cell line (<a href='/products/cell-lines/human-ret-knockout-mcf7-cell-line-ab286279'>ab286279</a>) at 20 µg

Lane 3:

THP-1 UNBOILED at 20 µg

Lane 4:

HepG2 UNBOILED at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 150 kDa,175 kDa

Observed band size: 150 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-SIRT2 antibody [EPR20411-105] ab211033 staining at 1/5000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 45 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in SIRT2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SIRT2 antibody [EPR20411-105] (<a href='/products/primary-antibodies/sirt2-antibody-epr20411-105-ab211033'>ab211033</a>) at 1/5000 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human SIRT2 knockout MCF7 cell line (ab289308) at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-SIRT2 antibody [EPR1667] ab134171 staining at 1/500 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 45 kDa in Wild-type MCF7 cell lysates with no signal observed at this size in SIRT2 knockout MCF7 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SIRT2 antibody [EPR1667] (<a href='/products/primary-antibodies/sirt2-antibody-epr1667-ab134171'>ab134171</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 at 20 µg

Lane 2:

Western blot - Human SIRT2 knockout MCF7 cell line (ab289308) at 20 µg

Lane 3:

THP-1 at 20 µg

Lane 4:

HeLa at 20 µg

Lane 5:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-TSC2 antibody [EP1107Y] (ab52936) staining at 1/20000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52936 detected a band observed at 200 kDa in wild-type MCF7 cell lysates with no change observed in the TSC2 knockout cell line ab286525. To generate this image, wild-type and TSC2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Tuberin antibody [EP1107Y] (<a href='/products/primary-antibodies/tuberin-antibody-ep1107y-ab52936'>ab52936</a>) at 1/20000 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human TSC2 knockout MCF7 cell line (<a href='/products/cell-lines/human-tsc2-knockout-mcf7-cell-line-ab286525'>ab286525</a>)

Lane 2:

TSC2 knockout MCF7 cell lysate at 20 µg

Lane 3:

Wild-type HAP1 cell lysate at 20 µg

Lane 4:

TSC2 knockout HAP1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 201 kDa

Observed band size: 200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CEACAM5 antibody (ab131070) staining at 0.1 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab131070 was shown to bind specifically to CEACAM5. A band was observed at 170 kDa in wild-type A549 cell lysates with no signal observed at this size in CEACAM5 knockout cell line. To generate this image, wild-type and CEACAM5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-CEACAM5 antibody (<a href='/products/primary-antibodies/carcino-embryonic-antigen-cea-antibody-ab131070'>ab131070</a>) at 0.1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

Western blot - Human CEACAM5 knockout A549 cell line (ab287319)

Lane 2:

CEACAM5 knockout A549 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

PANC-1 cell lysate at 20 µg

Predicted band size: 76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR4033] to BNIP3L/NIX ab109414 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 26-36 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-BNIP3L/NIX antibody [EPR4033] (<a href='/products/primary-antibodies/bnip3l-nix-antibody-epr4033-ab109414'>ab109414</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 0.1mM CoCl2 24h at 20 µg

Lane 2:

Wild-type A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 3:

BNIP3L knockout A549 0.1mM CoCl2 24h at 20 µg

Lane 4:

BNIP3L knockout A549 Vehicle Control 0mM CoCl2 24h at 20 µg

Lane 5:

HeLa 0.1mM CoCl2 24h at 20 µg

Lane 6:

HeLa Vehicle Control 0mM CoCl2 24h at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 24 kDa

Observed band size: 26-36 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[UDD3 30(12)] to LRRK2 ab133518 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 268 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in LRRK2 knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-LRRK2 antibody [UDD3 30(12)] (<a href='/products/primary-antibodies/lrrk2-antibody-udd3-3012-ab133518'>ab133518</a>) at 1/1000 dilution

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

Western blot - Human LRRK2 knockout U-87 MG cell line (ab306722) at 20 µg

Lane 3:

A549 at 20 µg

Lane 4:

HT-29 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 286 kDa

Observed band size: 268 kDa,80 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal [EPR23044-100] to RUNX1 / AML1 ab240639 staining at a 1/200 dilution, shown in black; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 50-54 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in RUNX1 knockout U-87 MG cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20,000 dilution.

Lanes 1 - 4:

Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] (<a href='/products/primary-antibodies/runx1-aml1-antibody-epr23044-100-ab240639'>ab240639</a>) at 1/200 dilution

Lanes 1 - 4:

Western blot - Anti-RUNX1 / AML1 antibody [EPR23044-100] - BSA and Azide free (<a href='/products/primary-antibodies/runx1-aml1-antibody-epr23044-100-bsa-and-azide-free-ab264471'>ab264471</a>) at 1/200 dilution

Lane 1:

Wild-type U-87 MG at 40 µg

Lane 2:

Western blot - Human RUNx1 knockout U-87 MG cell line (ab306773) at 40 µg

Lane 3:

Jurkat at 20 µg

Lane 4:

Caco-2 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 50-54 kDa

true

Exposure time: 10s

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-PHLPP1 antibody [EPR27151-55] ab305295 staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 160-200 kDa in Wild-type A549 cell lysates with no signal observed at this size in PHLPP1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-PHLPP1 antibody [EPR27151-55] (<a href='/products/primary-antibodies/phlpp1-antibody-epr27151-55-ab305295'>ab305295</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 40 µg

Lane 2:

Western blot - Human PHLPP1 knockout A549 cell line (<a href='/products/cell-lines/human-phlpp1-knockout-a549-cell-line-ab287700'>ab287700</a>) at 40 µg

Lane 3:

HEK-293 at 20 µg

Lane 4:

U-2 OS at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 185 kDa

Observed band size: 160-200 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-Chd7 antibody staining at 2 ug/ml, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab117522 was found to be non-specific. A band was observed at 160 kDa in wild-type SH-SY5Y cell lysates with no change observed in the CHD7 knockout cell line ab280067 (knockout cell lysate ab280126). To generate this image, wild-type and CHD7 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Chd7 antibody (<a href='/products/primary-antibodies/chd7-antibody-ab117522'>ab117522</a>) at 2 µg/mL

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

CHD7 knockout SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

DLD-1 cell lysate at 20 µg

Lane 5:

HEK-293 cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 5:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 336 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : HRP Anti-ALDH5A1/SSADH antibody [EPR7794] (ab314408) staining at 1/1000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab314408 was shown to bind specifically to ALDH5A1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibody Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 8 minutes exposure time.

All lanes:

Western blot - HRP Anti-ALDH5A1/SSADH antibody [EPR7794] (<a href='/products/primary-antibodies/hrp-aldh5a1-ssadh-antibody-epr7794-ab314408'>ab314408</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 20 µg

Lane 2:

Human liver lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 54 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

All lanes:

Anti-USP18 antibody at 1/1000 dilution

Lane 1:

Wild-type A549 Vehicle Control IFNa (0 IU/mL, 12 h) cell lysate at 20 µg

Lane 2:

Wild-type A549 Treated IFNa (100 IU/mL, 12 h) cell lysate at 20 µg

Lane 3:

USP18 knockout A549 Vehicle Control IFNa (0 IU/mL, 12 h) ab262530 cell lysate at 20 µg

Lane 4:

USP18 knockout A549 Treated IFNa (100 IU/mL, 12 h) ab262530 cell lysate at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 43 kDa

Observed band size: 34-37 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-DCR2 antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 53 kDa in Wild-type A549 UNBOILED cell lysates with no signal observed at this size in TNFRSF10D knockout A549 UNBOILED cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Anti-DCR2 antibody at 1/1000 dilution

Lane 1:

Wild-type A549 UNBOILED at 20 µg

Lane 2:

TNFRSF10D knockout A549 UNBOILED at 20 µg

Lane 3:

U-87 MG UNBOILED at 20 µg

Lane 4:

Caco-2 UNBOILED at 20 µg

Lane 5:

Raji UNBOILED at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 53 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-Chd7 antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab176807 was found to be non-specific. A band was observed at 160 kDa in wild-type SH-SY5Y cell lysates with no change observed in the CHD7 knockout cell line ab280067 (knockout cell lysate ab280126). To generate this image, wild-type and CHD7 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Chd7 antibody (<a href='/products/primary-antibodies/chd7-antibody-ab176807'>ab176807</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 20 µg

Lane 2:

CHD7 knockout SH-SY5Y cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

DLD-1 cell lysate at 20 µg

Lane 5:

HEK-293 cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution

Lanes 1 - 5:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 336 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-Lipin 1 antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 122 kDa in Wild-type A549 cell lysates with no signal observed at this size in LPIN1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Anti-Lipin 1 antibody at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human LPIN1 knockout A549 cell line (ab301047) at 20 µg

Lane 2:

LPIN1 knockout A549 cell line at 20 µg

Lane 3:

K562 at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 99 kDa,130 kDa

Observed band size: 122 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Polyclonal to AMACR ab246927 staining at 0.4 µg/mL, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type U-87 MG cell lysates with no signal observed at this size in AMACR knockout U-87 MG cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-AMACR antibody (<a href='/products/primary-antibodies/amacr-antibody-ab246927'>ab246927</a>) at 0.4 µg/mL

Lane 1:

Wild-type U-87 MG at 20 µg

Lane 2:

AMACR knockout U-87 MG at 20 µg

Lane 3:

LNCaP at 20 µg

Lane 4:

Raji at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 40 kDa,77 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-PRKDC antibody [EPR392] (ab133516) staining at 1/2000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133516 was shown to bind specifically to PRKDC. A band was observed at 450 kDa in wild-type A549 cell lysates with no signal observed at this size in PRKDC knockout cell line. To generate this image, wild-type and PRKDC knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-DNA PKcs antibody [EPR392] (<a href='/products/primary-antibodies/dna-pkcs-antibody-epr392-ab133516'>ab133516</a>) at 1/2000 dilution

Lanes 1 - 4:

Western blot at 20 µg

Lane 2:

Western blot - Human PRKDC knockout A549 cell line (<a href='/products/cell-lines/human-prkdc-knockout-a549-cell-line-ab276100'>ab276100</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 469 kDa

Observed band size: 450 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-FLT4 antibody [EPR22293-14] (ab243232) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243232 was shown to bind specifically to FLT4. First, unboiled samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-VEGF Receptor 3 antibody [EPR22293-14] (<a href='/products/primary-antibodies/vegf-receptor-3-antibody-epr22293-14-ab243232'>ab243232</a>) at 1/1000 dilution

Lane 1:

HEL 92.1.7 cell lysate at 20 µg

Lane 2:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 146 kDa

Observed band size: 200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

All lanes:

Western blot - Anti-ATP7b antibody [EPR6794] (<a href='/products/primary-antibodies/atp7b-antibody-epr6794-ab124973'>ab124973</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 10 µg

Lane 2:

ATP7B knockout A549 at 10 µg

Lane 3:

HepG2 at 10 µg

Lane 4:

Ramos at 10 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 157 kDa

Observed band size: 153 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-SMAD5 antibody [EP619Y] (ab40771) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab40771 was shown to bind specifically to SMAD5. A band was observed at 52 kDa in wild-type HeLa cell lysates with no signal observed at this size in SMAD5 knockout cell line. To generate this image, wild-type and SMAD5 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SMAD5 antibody [EP619Y] (<a href='/products/primary-antibodies/smad5-antibody-ep619y-ab40771'>ab40771</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa Vehicle Control TGF-beta (0 ng/mL, 30 min) cell lysate at 20 µg

Lane 2:

Wild-type HeLa Treated TGF-beta (10 ng/mL, 30 min) cell lysate at 20 µg

Lane 3:

SMAD1 knockout HeLa Vehicle Control TGF-beta (0 ng/mL, 30 min), <a href='/products/cell-lines/human-smad1-knockout-hela-cell-line-ab265400'>ab265400</a> cell lysate at 20 µg

Lane 4:

SMAD1 knockout HeLa Treated TGF-beta (10 ng/mL, 30 min), <a href='/products/cell-lines/human-smad1-knockout-hela-cell-line-ab265400'>ab265400</a> cell lysate at 20 µg

Lane 5:

SMAD2 knockout HeLa Vehicle Control TGF-beta (0 ng/mL, 30 min), <a href='/products/cell-lines/human-smad2-knockout-hela-cell-line-ab255430'>ab255430</a> cell lysate at 20 µg

Lane 6:

SMAD2 knockout HeLa Treated TGF-beta (10 ng/mL, 30 min), <a href='/products/cell-lines/human-smad2-knockout-hela-cell-line-ab255430'>ab255430</a> cell lysate at 20 µg

Lane 7:

Wild-type HeLa Vehicle Control TGF-beta (0 ng/mL, 30 min), ab255448 cell lysate at 20 µg

Lane 8:

Wild-type HeLa Treated TGF-beta (10 ng/mL, 30 min), ab255448 cell lysate at 20 µg

Lane 9:

SMAD3 knockout HeLa Vehicle Control TGF-beta (0 ng/mL, 30 min), <a href='/products/cell-lines/human-smad3-knockout-hela-cell-line-ab255431'>ab255431</a> cell lysate at 20 µg

Lane 10:

SMAD3 knockout HeLa Treated TGF-beta (10 ng/mL, 30 min), <a href='/products/cell-lines/human-smad3-knockout-hela-cell-line-ab255431'>ab255431</a> cell lysate at 20 µg

Lane 11:

Wild-type HEK293 Vehicle Control TGF-beta (0 ng/mL, 30 min), ab259776 cell lysate at 20 µg

Lane 12:

Wild-type HEK293 Treated TGF-beta (10 ng/mL, 30 min), ab259776 cell lysate at 20 µg

Lane 13:

SMAD5 knockout HEK293 Vehicle Control TGF-beta (0 ng/mL, 30 min), <a href='/products/cell-lines/human-smad5-knockout-hek-293-cell-line-ab269470'>ab269470</a> cell lysate at 20 µg

Lane 14:

SMAD5 knockout HEK293 Treated TGF-beta (10 ng/mL, 30 min), <a href='/products/cell-lines/human-smad5-knockout-hek-293-cell-line-ab269470'>ab269470</a> cell lysate at 20 µg

Secondary

Lanes 1 - 14:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 14:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 52 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-CP antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 140-150 kDa. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc BSA in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Ceruloplasmin (D7Q5W) Rabbit mAb at 1/1000 dilution

Lane 1:

Wild-type A549 Treated BFA (5ug/mL, 6h) at 20 µg

Lane 2:

Wild-type A549 Vehicle Control BFA (0ug/mL, 6h) at 20 µg

Lanes 3 - 4:

Western blot - Human CP Knockout A549 cell line (ab314964) at 20 µg

Lane 5:

SK-OV-3 at 20 µg

Lane 6:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 122 kDa

Observed band size: 140-150 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-Perilipin 2 antibody ab78920 staining at 1 µg/mL, shown in green; Mouse anti-CANX ab238078 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type A549 cell lysates with no signal observed at this size in PLIN2 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Perilipin 2 antibody (<a href='/products/primary-antibodies/perilipin-2-antibody-ab78920'>ab78920</a>) at 1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

PLIN2 knockout A549 at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

SK-OV-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 55 kDa,95 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Lanes 1 - 4 : Merged signal (red and green). Green - ab238078 observed at 80 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab238078 was shown to react with Calnexin in wild-type HEK-293T cells in Western blot with loss of signal observed in CANX knockout cell line ab255368 (CANX knockout cell lysate ab263805). Wild-type HEK-293T and CANX knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab238078 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Calnexin antibody [CANX/1543] (ab238078) at 1 µg/mL

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

CANX knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human CANX (Calnexin) knockout HEK-293T cell line (<a href='/products/cell-lines/human-canx-calnexin-knockout-hek-293t-cell-line-ab255368'>ab255368</a>)

Lane 3:

U-2 OS cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Predicted band size: 68 kDa

Observed band size: 80 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Polyclonal to Glycine decarboxylase ab204087 staining at 0.4 µg/mL, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 110 kDa in Wild-type A549 cell lysates with no signal observed at this size in GLDC knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Glycine decarboxylase antibody (<a href='/products/primary-antibodies/glycine-decarboxylase-antibody-ab204087'>ab204087</a>) at 0.4 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human GLDC knockout A549 cell line (<a href='/products/cell-lines/human-gldc-knockout-a549-cell-line-ab300972'>ab300972</a>) at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

HAP1 at 20 µg

Lane 5:

Raji at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-PLCG2 antibody [EPR5914-34] (ab133522) staining at 1/10000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133522 was shown to bind specifically to PLCG2. A band was observed at 148 kDa in wild-type HAP1 cell lysates with no signal observed at this size in PLCG2 knockout cell line. To generate this image, wild-type and PLCG2 knockout HAP1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-PLCG 2 antibody [EPR5914-34] (<a href='/products/primary-antibodies/plcg-2-antibody-epr5914-34-ab133522'>ab133522</a>) at 1/10000 dilution

All lanes:

Western blot at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 148 kDa

Observed band size: 148 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-LDLR antibody [EPR24874-56] (ab271189) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab271189 was shown to bind specifically to LDLR. A band was observed at 120, 150 kDa in wild-type HeLa cell lysates with no signal observed at this size in LDLR CRISPR-Cas9 edited cell line ab273838 (CRISPR-Cas9 edited cell lysate ab273792). The band observed in the CRISPR-Cas9 edited lysate lane below 120, 150 kDa is likely to represent a possible truncated form of LDLR. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and LDLR CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween®20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-LDL Receptor antibody [EPR24874-56] (<a href='/products/primary-antibodies/ldl-receptor-antibody-epr24874-56-ab271189'>ab271189</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human LDLR (LDL Receptor) knockout HeLa cell line (<a href='/products/cell-lines/human-ldlr-ldl-receptor-knockout-hela-cell-line-ab273838'>ab273838</a>)

Lane 2:

LDLR CRISPR-Cas9 edited HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

A431 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 120 kDa,150 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-IGF1R antibody [EPR23027-204] (ab263903) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab263903 was shown to bind specifically to IGF1R. A band was observed at 105 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in IGF1R knockout cell line. To generate this image, wild-type and IGF1R knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-IGF1 Receptor antibody [EPR23027-204] (<a href='/products/primary-antibodies/igf1-receptor-antibody-epr23027-204-ab263903'>ab263903</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot at 20 µg

Lane 2:

Western blot - Human IGF1R knockout HCT116 cell line (<a href='/products/cell-lines/human-igf1r-knockout-hct116-cell-line-ab287509'>ab287509</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 154 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Anti-HERPUD1 antibody [EPR9649] ab150424 staining at 1/1000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 49 kDa in Wild-type A549 cell lysates with no signal observed at this size in HERPUD1 knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-HERPUD1 antibody [EPR9649] (<a href='/products/primary-antibodies/herpud1-antibody-epr9649-ab150424'>ab150424</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

HERPUD1 knockout A549 at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 49 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Western blot : Rabbit Monoclonal[EPR3536(2)] to CAMK1D ab172618 staining at 1/10000 dilution, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 42 kDa in Wild-type A549 cell lysates with no signal observed at this size in CAMK1D knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-CAMK1D antibody [EPR3536(2)] (<a href='/products/primary-antibodies/camk1d-antibody-epr35362-ab172618'>ab172618</a>) at 1/10000 dilution

Lane 1:

Wild-type A549 at 10 µg

Lane 2:

CAMK1D knockout A549 at 10 µg

Lane 3:

HeLa at 10 µg

Lane 4:

K562 at 10 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa,76 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-Insulin Receptor antibody staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137747 was shown to bind specifically to Insulin Receptor. A band was observed at 85/200 kDa in wild-type HepG2 cell lysates with no signal observed at this size in INSR knockout cell line ab282827 (knockout cell lysate ab283051). Pro-form of INSR is observed at 200 kDa and the mature form observed at 90 kDa. To generate this image, wild-type and INSR knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Insulin Receptor antibody (<a href='/products/primary-antibodies/insulin-receptor-antibody-ab137747'>ab137747</a>) at 1/1000 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human INSR knockout Hep G2 cell lysate (ab283051)

Lane 2:

INSR knockout HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

HDLM-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 156 kDa

Observed band size: 85 kDa,200 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-SOS1 antibody [EPR7480] (ab140621) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 165 kDa in wild-type A549 cell lysates with no signal observed at this size in SOS1 knockout cell line. To generate this image, wild-type and SOS1 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-SOS1 antibody [EPR7480] (<a href='/products/primary-antibodies/sos1-antibody-epr7480-ab140621'>ab140621</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot at 20 µg

Lane 2:

Western blot - Human SOS1 knockout A549 cell line (<a href='/products/cell-lines/human-sos1-knockout-a549-cell-line-ab286377'>ab286377</a>)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 152 kDa

Observed band size: 165 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-NF1 antibody [EPR22989-68] (ab238142) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab238142 was shown to bind specifically to NF1. A band was observed at 180-270 kDa in wild-type MCF7 cell lysates with no signal observed at this size in NF1 knockout cell line. To generate this image, wild-type and NF1 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-Neurofibromin antibody [EPR22989-68] (<a href='/products/primary-antibodies/neurofibromin-antibody-epr22989-68-ab238142'>ab238142</a>) at 1/1000 dilution

Lanes 1 - 4:

Western blot at 20 µg

Lane 2:

Western blot - Human NF1 knockout MCF7 cell line (<a href='/products/cell-lines/human-nf1-knockout-mcf7-cell-line-ab286416'>ab286416</a>) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 319 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

Anti-BRCA2 antibody [EPR23442-43] (ab239375) staining at 1/500 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab239375 was shown to bind specifically to BRCA2. A band was observed at 290 kDa in wild-type MCF7 cell lysates with no signal observed at this size in BRCA2 knockout cell line ab286285 (knockout cell lysate ab300210). To generate this image, wild-type and BRCA2 knockout MCF7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

All lanes:

Western blot - Anti-BRCA2 antibody [EPR23442-43] (<a href='/products/primary-antibodies/brca2-antibody-epr23442-43-ab239375'>ab239375</a>) at 1/500 dilution

Lane 1:

Wild-type MCF7 cell lysate at 20 µg

Lane 2:

Western blot - Human BRCA2 knockout MCF7 cell lysate (ab300210) at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Lane 5:

HT-29 cell lysate at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit IgG H&L 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 290 kDa

false

• WB

Lab

Western blot - Anti-Calnexin antibody [CANX/1543] (AB238078)

False colour image of Western blot : Anti-DNA PKcs antibody [Y393] staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32566 was shown to bind specifically to DNA PKcs. A band was observed at 450 kDa in wild-type A549 cell lysates with no signal observed at this size in PRKDC knockout cell line. To generate this image, wild-type and PRKDC knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-DNA PKcs antibody [Y393] (<a href='/products/primary-antibodies/dna-pkcs-antibody-y393-ab32566'>ab32566</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

PRKDC knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human PRKDC knockout A549 cell line (<a href='/products/cell-lines/human-prkdc-knockout-a549-cell-line-ab276100'>ab276100</a>)

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

HDLM-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 469 kDa

Observed band size: 450 kDa

false