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AB219644

Anti-Calnexin抗体- C-terminal

Anti-Calnexin antibody - C-terminal

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(8 Publications)

Anti-Calnexin antibody - C-terminal (ab219644) is a goat polyclonal antibody detecting Calnexin in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.

查看别名

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (AB219644)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - C-terminal (AB219644)

Immunofluorescent analysis of Hepa1-6 cells (4% of PFA-fixed) labeling Calnexin with ab219644 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling Calnexin with ab219644 at 5 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - C-terminal (AB219644)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling Calnexin with ab219644 at 5 μg/ml.

关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

IHC-P, ICC/IF, WB

applications

免疫原

Recombinant Fragment Protein within Human CANX aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P27824

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替代产品

Primary Antibodies

AB241154

Anti-Calnexin antibody [EPR21240] - ER Membrane Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Calnexin antibody [EPR21240] - ER Membrane Marker (AB241154)

  • 0
  • 0
Primary Antibodies

AB213243

Anti-Calnexin antibody [EPR21205]

20ul selling size|Recombinant|KO Validated

Western blot - Anti-Calnexin antibody [EPR21205] (AB213243)

  • 0
  • 0
Primary Antibodies

AB195198

HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB195198)

  • 0
  • 0
Primary Antibodies

AB92573

Anti-Calnexin antibody [EPR3632]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR3632] (AB92573)

  • 0
  • 1
Primary Antibodies

AB133615

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB133615)

  • 5
  • 1
Primary Antibodies

AB203439

Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

  • 0
  • 0
Primary Antibodies

AB232433

Anti-Calnexin antibody [EPR3632] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR3632] - BSA and Azide free (AB232433)

  • 0
  • 1
Primary Antibodies

AB225542

Anti-Calnexin antibody [EPR3633(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Calnexin antibody [EPR3633(2)] - BSA and Azide free (AB225542)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB79559

Anti-CD81 antibody [M38]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD81 antibody [M38] (AB79559)

  • 4
  • 2
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/500", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

What is this antibody validated in?
Anti-Calnexin antibody - C-terminal (ab219644) is a goat polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

What is the molecular weight of Calnexin?
Anti-Calnexin - C-terminal (ab219644) specifically detects a band for Calnexin (UniProt: P27824) at a molecular weight of 68kDa.
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 20% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
See full target information CANX
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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7035 PubMed40745160

2025

Mogat1 drives metabolic adaptations to evade immune surveillance.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wei,Congyi Niu,Yue Shi,Yingfei Fang,Chengheng Yang,Jian Liu,Zhenjie Xu

Nature communications 16:4393 PubMed40355429

2025

SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Stella Hartmann,Lisa Radochonski,Chengjin Ye,Luis Martinez-Sobrido,Jueqi Chen

The Journal of biological chemistry 301:108292 PubMed39952409

2025

Nanobody fusion enhances production of difficult-to-produce secretory proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Runchuan Yan,Yan Zhang,Hui Zhang,Jiyan Ma

Cell death & disease 15:771 PubMed39443481

2024

Enhanced mGluR intracellular activity causes psychiatric alterations in Niemann Pick type C disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Toledano-Zaragoza,Violeta Enriquez-Zarralanga,Sara Naya-Forcano,Víctor Briz,Rocío Alfaro-Ruíz,Miguel Parra-Martínez,Daniel N Mitroi,Rafael Luján,José A Esteban,María Dolores Ledesma

JCI insight 8: PubMed37014698

2023

Polarized localization of phosphatidylserine in the endothelium regulates Kir2.1.

Applications

Unspecified application

Species

Unspecified reactive species

Claire A Ruddiman,Richard Peckham,Melissa A Luse,Yen-Lin Chen,Maniselvan Kuppusamy,Bruce A Corliss,P Jordan Hall,Chien-Jung Lin,Shayn M Peirce,Swapnil K Sonkusare,Robert P Mecham,Jessica E Wagenseil,Brant E Isakson

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e2105320 PubMed35748162

2022

TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike.

Applications

Unspecified application

Species

Unspecified reactive species

Hak Park,Soo Kyung Seo,Ju-Ri Sim,Su Jin Hwang,Ye Jin Kim,Dong Hoon Shin,Dong Geon Jang,Shin Hye Noh,Pil-Gu Park,Si Hwan Ko,Mi Hwa Shin,Jae Young Choi,Yukishige Ito,Chung-Min Kang,Jae Myun Lee,Min Goo Lee

mBio 13:e0271721 PubMed35038927

2022

Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-An Kung,Huan-Jung Chiang,Mei-Ling Li,Yu-Nong Gong,Hsin-Ping Chiu,Chuan-Tien Hung,Peng-Nien Huang,Sheng-Yu Huang,Pei-Yu Wang,Tsu-An Hsu,Gary Brewer,Shin-Ru Shih

Nature communications 10:2914 PubMed31266968

2019

The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tongde Du,Hongchang Li,Yongsheng Fan,Lin Yuan,Xiaodan Guo,Qiong Zhu,Yuying Yao,Xin Li,Chunlei Liu,Xinhe Yu,Zhaofei Liu,Chun-Ping Cui,Chuanchun Han,Lingqiang Zhang
View all publications
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