Anti-Calnexin抗体(ab10286)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calnexin抗体
参阅全部 Calnexin 一抗 -
描述
兔多克隆抗体to Calnexin -
宿主
Rabbit -
特异性
Recognizes ER membrane, mitochondria and cis-Golgi -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (His-tag) corresponding to Dog Calnexin aa 1-500. Expressed in E. coli. N-terminal ER luminal domain.
Database link: P24643 -
阳性对照
- WB: Human CEM T-lymphoblastoid leukemia cell lines. MCF cells. HeLa total cell lysate. HeLa membrane fraction. MDA-MB231 total cell lysate. MDA-MB231 membrane fraction.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: 99% Rabbit Serum -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab10286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (14) |
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.
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| 说明 |
|---|
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WB
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. |
靶标
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功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 821 Human
- Omim: 114217 Human
- SwissProt: P27824 Human
- Unigene: 567968 Human
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别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
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Western blot - Anti-Calnexin antibody (ab10286)
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines. -
Western blot - Anti-Calnexin antibody (ab10286)Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size: 67 kDa -
Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Dr Neil TaylorAll lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 5 minutes
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Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an anonymous Abreview.Anti-Calnexin antibody (ab10286) at 1/2000 dilution + HeLa whole cell lysate
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
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Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : CHO membrane fraction
Lane 2 : CHO cytosolic fraction
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution
Predicted band size: 67 kDa
数据表及文件
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Datasheet download
文献 (83)
ab10286 被引用在 83 文献中.
- Aharon A et al. Associations of maternal and placental extracellular vesicle miRNA with preeclampsia. Front Cell Dev Biol 11:1080419 (2023). PubMed: 36910147
- Bauer FN et al. CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities. Cytotherapy 25:138-147 (2023). PubMed: 36244910
- Hara M et al. PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle. Cell Rep 42:112091 (2023). PubMed: 36763501
- Qian K et al. CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization. Nature 613:160-168 (2023). PubMed: 36477540
- Veerman RE et al. Antigen-Loaded Extracellular Vesicles Induce Responsiveness to Anti-PD-1 and Anti-PD-L1 Treatment in a Checkpoint Refractory Melanoma Model. Cancer Immunol Res 11:217-227 (2023). PubMed: 36546872
