AB10286

Anti-Calnexin抗体

Name: Calnexin抗体 (ab10286)| Abcam中文官网
Brand: Abcam Limited
SKU: AB10286
Rating: 4 (21 reviews)

Anti-Calnexin antibody

(21 Reviews)

(84 Publications)

Rabbit Polyclonal Calnexin antibody. Suitable for WB and reacts with Human samples. Cited in 84 publications. Immunogen corresponding to Recombinant Fragment Protein within Dog CANX aa 1-500.

查看别名

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

5 Images

Unknown

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/1000 dilution

All lanes:

MCF7 cells

Secondary

All lanes:

Goat anti rabbit at 1/10000 dilution

Predicted band size: 68 kDa

false

AbReview21557****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/2000 dilution

All lanes:

HeLa whole cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

true

This image is courtesy of an anonymous Abreview.

Unknown

Western blot - Anti-Calnexin antibody (AB10286)

Western blot probed with ab10286 at 1/1000.

Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.

Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.

All lanes:

Western blot - Anti-Calnexin antibody (ab10286)

Predicted band size: 68 kDa

false

AbReview7867****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/4000 dilution

Lane 1:

HeLa (Total cell lysate)

Lane 2:

HeLa (Membrane fraction)

Lane 3:

HeLa (Cytoplasmic fraction)

Lane 4:

MDA-MB231 (Total cell lysate)

Lane 5:

MDA-MB231 (Membrane fraction)

Lane 6:

MDA-MB231 (Cytoplasmic fraction)

Secondary

All lanes:

HRP conjugated donkey anti-rabbit

Predicted band size: 68 kDa

Observed band size: 67 kDa

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Dr Neil Taylor

AbReview24921****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/4000 dilution

Lane 1:

CHO membrane fraction

Lane 2:

CHO cytosolic fraction

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution

Predicted band size: 68 kDa

false

This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Recombinant Fragment Protein within Dog CANX aa 1-500. The exact immunogen used to generate this antibody is proprietary information.

P24643

特异性

Recognizes ER membrane, mitochondria and cis-Golgi

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p>1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.</p>" } } }

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您是否考虑以下替代产品？

AB241154

Anti-Calnexin antibody [EPR21240] - ER Membrane Marker

0 Reviews

查看产品

为什么推荐这个？

为向您提供最高品质的产品，我们在不断升级产品范围。我们选择了这个替代产品供您参考，因为它提供了更好的性能，更容易和更方便的使用，或交期更快。

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性能和储存信息

形式

Liquid

纯度

Whole antiserum

存储溶液

Constituents: 99% Rabbit Serum

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.

Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.

See full target information CANX

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文献 (84)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 11: PubMed37626791

2023

Circulating and Exosomal microRNA-33 in Childhood Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cabiati,Letizia Guiducci,Emioli Randazzo,Valentina Casieri,Giovanni Federico,Silvia Del Ry

Theranostics 13:3707-3724 PubMed37441602

2023

Extracellular vesicles improve GABAergic transmission in Huntington's disease iPSC-derived neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Margarida Beatriz,Ricardo J Rodrigues,Rita Vilaça,Conceição Egas,Paulo S Pinheiro,George Q Daley,Thorsten M Schlaeger,Nuno Raimundo,A Cristina Rego,Carla Lopes

Frontiers in cell and developmental biology 11:1080419 PubMed36910147

2023

Associations of maternal and placental extracellular vesicle miRNA with preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Anat Aharon,Annie Rebibo-Sabbah,Rawan Sayed Ahmad,Ayelet Dangot,Tali Hana Bar-Lev,Benjamin Brenner,Adi Halberthal Cohen,Chen Ben David,Zeev Weiner,Ido Solt

Communications biology 6:208 PubMed36813870

2023

Soluble Klotho protects against glomerular injury through regulation of ER stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Emmanuelle Charrin,Dina Dabaghie,Ilke Sen,David Unnersjö-Jess,Katja Möller-Hackbarth,Mikhail Burmakin,Rik Mencke,Sonia Zambrano,Jaakko Patrakka,Hannes Olauson

Cell reports 42:112091 PubMed36763501

2023

PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Miwa Hara,Wenjing Wu,Volha V Malechka,Yusuke Takahashi,Jian-Xing Ma,Gennadiy Moiseyev

Laboratory investigation; a journal of technical methods and pathology 101:837-850 PubMed36775555

2023

Extracellular vesicles derived from M2 microglia reduce ischemic brain injury through microRNA-135a-5p/TXNIP/NLRP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Liu,You-Ping Li,Li-Min Xiao,Li-Ke Chen,Su-Yue Zheng,Er-Ming Zeng,Chun-Hua Xu

Cancer immunology research 11:217-227 PubMed36546872

2022

Antigen-Loaded Extracellular Vesicles Induce Responsiveness to Anti-PD-1 and Anti-PD-L1 Treatment in a Checkpoint Refractory Melanoma Model.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanne E Veerman,Gözde Güclüler Akpinar,Annemarijn Offens,Loïc Steiner,Pia Larssen,Andreas Lundqvist,Mikael C I Karlsson,Susanne Gabrielsson

International journal of molecular sciences 24: PubMed36613584

2022

Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cabiati,Emioli Randazzo,Letizia Guiducci,Alessandra Falleni,Antonella Cecchettini,Valentina Casieri,Giovanni Federico,Silvia Del Ry

Nature 613:160-168 PubMed36477540

2022

CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Qian,Marcus J Tol,Jin Wu,Lauren F Uchiyama,Xu Xiao,Liujuan Cui,Alexander H Bedard,Thomas A Weston,Pradeep S Rajendran,Laurent Vergnes,Yuta Shimanaka,Yesheng Yin,Yasaman Jami-Alahmadi,Whitaker Cohn,Bryce T Bajar,Chia-Ho Lin,Benita Jin,Laura A DeNardo,Douglas L Black,Julian P Whitelegge,James A Wohlschlegel,Karen Reue,Kalyanam Shivkumar,Feng-Jung Chen,Stephen G Young,Peng Li,Peter Tontonoz

Cytotherapy 25:138-147 PubMed36244910

2022

CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities.

Applications

Unspecified application

Species

Unspecified reactive species

Fabiola Nardi Bauer,Tobias Tertel,Oumaima Stambouli,Chen Wang,Robin Dittrich,Simon Staubach,Verena Börger,Dirk M Hermann,Sven Brandau,Bernd Giebel

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

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primary-antibodies

calnexin-antibody-epr36332-bsa-and-azide-free-ab225542

(0 reviews)

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Calnexin抗体

Anti-Calnexin antibody

Western blot - Anti-Calnexin antibody (AB10286)

Western blot - Anti-Calnexin antibody (AB10286)

Western blot - Anti-Calnexin antibody (AB10286)

Western blot - Anti-Calnexin antibody (AB10286)

Western blot - Anti-Calnexin antibody (AB10286)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性

反应性数据

您是否考虑以下替代产品？

Anti-Calnexin antibody [EPR21240] - ER Membrane Marker

为什么推荐这个？

性能和储存信息

形式

纯度

存储溶液

运输条件

推荐的短期储存时间

推荐的短期储存条件

推荐的长期储存条件

分装信息

储存信息

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (84)

Abcam Product Promise

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