Anti-Calmodulin 1/2/3 (phospho T79 + S81)抗体(ab61001)
Key features and details
- Rabbit polyclonal to Calmodulin 1/2/3 (phospho T79 + S81)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Calmodulin 1/2/3 (phospho T79 + S81)抗体
参阅全部 Calmodulin 1/2/3 一抗 -
描述
兔多克隆抗体to Calmodulin 1/2/3 (phospho T79 + S81) -
宿主
Rabbit -
特异性
Detects endogenous levels of Calmodulin only when phosphorylated at threonine 79 and serine 81. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Calmodulin 1/2/3 (phospho T79 + S81). The antiserum was produced against synthesized phosphopeptide derived from human Calmodulin around the phosphorylation site of threonine 79 and serine 81 (K-D-T(p)-D-S(p)-E-E)
Database link: P0DP23 -
阳性对照
- IHC-P: human brain tissue. WB: extracts from Jurkat cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Affinity purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61001于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/100 - 1/500.
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| WB |
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
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| IHC-P |
1/50 - 1/100.
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| 说明 |
|---|
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ICC/IF
1/100 - 1/500. |
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WB
1/500 - 1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa). |
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IHC-P
1/50 - 1/100. |
靶标
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相关性
Function: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin-Ca(2+) complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. -
细胞定位
Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. -
数据库链接
- Entrez Gene: 808 Human
- Entrez Gene: 12315 Mouse
- Entrez Gene: 24244 Rat
- Omim: 114182 Human
- Omim: 114183 Human
- SwissProt: 114180 Human
- SwissProt: P0DP23 Human
- SwissProt: P0DP24 Human
see all -
形式
There are three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. -
别名
- CALM 1 antibody
- CALM 2 antibody
- CALM 3 antibody
see all
图片
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Western blot - Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001)All lanes : Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001) at 1/500 dilution
Lane 1 : HeLa cells treated with Hu (1mg/ml)
Lane 2 : Raw264.7 cells treated with TNFa (1mg/ml)
Lysates/proteins at 15 µl per lane.
Predicted band size: 17 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001)ab61001, at a 1/50 dilution, staining bi-phosphorylated Calmodulin on human brain tissue sections, in the presence (right) or absence (left) of the immunizing phosphopeptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Calmodulin 1/2/3 (phospho T79 + S81) antibody (ab61001)Immunofluorescence analysis of HepG2 cells labeling Calmodulin (Phospho-Thr79+Ser81) with ab61001 at 1/100 dilution. The panel on the left is without the blocking peptide, while the panel on the right includes the blocking peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab61001 被引用在 4 文献中.
- Hamilton S et al. PKA phosphorylation underlies functional recruitment of sarcolemmal SK2 channels in ventricular myocytes from hypertrophic hearts. J Physiol N/A:N/A (2019). PubMed: 30771223
- Hayrabedyan S et al. Synthetic PreImplantation Factor (sPIF) induces posttranslational protein modification and reverses paralysis in EAE mice. Sci Rep 9:12876 (2019). PubMed: 31578341
- Pachuau J et al. Protein kinase CK2 contributes to diminished small conductance Ca2+-activated K+ channel activity of hypothalamic pre-sympathetic neurons in hypertension. J Neurochem 130:657-67 (2014). PubMed: 24806793
- Seo HS et al. Phytoestrogens induce apoptosis via extrinsic pathway, inhibiting nuclear factor-kappaB signaling in HER2-overexpressing breast cancer cells. Anticancer Res 31:3301-13 (2011). PubMed: 21965740