Anti-Calcineurin抑制剂抗体(ab3349)
Key features and details
- Rabbit polyclonal to Calcineurin inhibitor
- Suitable for: ICC/IF
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Calcineurin抑制剂抗体 -
描述
兔多克隆抗体to Calcineurin抑制剂 -
宿主
Rabbit -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide (His-tag) corresponding to Rat Calcineurin inhibitor aa 1850 to the C-terminus.
Database link: O88480 -
阳性对照
- ICC/IF: PC12 and NRK cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Constituent: 99% PBS -
Concentration information loading... -
纯度
Whole antiserum -
Primary antibody说明
Calcineurin is a widely distributed Ser/Thr, calcium and calmodulin-dependent protein phosphatase. It mediates the immunosuppressive actions of drugs such as FK506 and cyclosporin, and has also been implicated in a number of calcium-sensitive pathways in the nervous system. Calcineurin has been found to be associated with other proteins, such as calmodulin, FKBP12, the ryanodine receptor, inositol 1,4,5-trisphosphate, and a recently identified protein, calcineurin inhibitor (CAIN). CAIN has been shown to bind calcineurin through its C-terminal tail. Recent studies have shown CAIN to bind to both amphiphysin 1 and calcineurin simultaneously and therefore acts as a physiological inhibitor of calcineurin. The binding of CAIN to amphiphysin 1 does not affect the interaction of amphiphysin 1 with other endocytic proteins. CAIN, like calcineurin, is highly expressed in neurons and other tissues. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/1000.
|
| 说明 |
|---|
|
ICC/IF
1/1000. |
靶标
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功能
May be required for replication-independent chromatin assembly. May serve as a negative regulator of T-cell receptor (TCR) signaling via inhibition of calcineurin. Inhibition of activated calcineurin is dependent on both PKC and calcium signals. -
组织特异性
Widely expressed in different tissues. -
序列相似性
Contains 6 TPR repeats. -
翻译后修饰
Activated through PKC-mediated hyperphosphorylation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 94165 Rat
- SwissProt: O88480 Rat
- Unigene: 4 Rat
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别名
- A330070M20Rik antibody
- Cabin 1 antibody
- CABIN_HUMAN antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)Immunocytochemistry/ Immunofluorescence analysis of NRK cells labeling Calcineurin inhibitor with ab3349. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS for 30 minutes at room temperature. Cells were stained with ab3349 at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate at a dilution of 1/1000 for 1 hour at room temperature (green). Nuclei (blue) were stained with Hoechst 33342 dye.
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Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin inhibitor antibody (ab3349)ICC/IF image of ab3349 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3349, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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Datasheet download
文献 (9)
ab3349 被引用在 9 文献中.
- Smith R et al. HIRA contributes to zygote formation in mice and is implicated in human 1PN zygote phenotype. Reproduction 161:697-707 (2021). PubMed: 33835048
- Hoang SM et al. Regulation of ALT-associated homology-directed repair by polyADP-ribosylation. Nat Struct Mol Biol 27:1152-1164 (2020). PubMed: 33046907
- Torné J et al. Two HIRA-dependent pathways mediate H3.3 de novo deposition and recycling during transcription. Nat Struct Mol Biol 27:1057-1068 (2020). PubMed: 32895554
- Zhang L et al. Downregulation of miRNA-15a and miRNA-16 promote tumor proliferation in multiple myeloma by increasing CABIN1 expression. Oncol Lett 15:1287-1296 (2018). PubMed: 29399181
- Ray-Gallet D et al. Functional activity of the H3.3 histone chaperone complex HIRA requires trimerization of the HIRA subunit. Nat Commun 9:3103 (2018). PubMed: 30082790
- Lee JS & Zhang Z O-linked N-acetylglucosamine transferase (OGT) interacts with the histone chaperone HIRA complex and regulates nucleosome assembly and cellular senescence. Proc Natl Acad Sci U S A 113:E3213-20 (2016). PubMed: 27217568
- Ying CY et al. MEF2B mutations lead to deregulated expression of the oncogene BCL6 in diffuse large B cell lymphoma. Nat Immunol 14:1084-92 (2013). PubMed: 23974956
- Rai TS et al. Human CABIN1 is a functional member of the human HIRA/UBN1/ASF1a histone H3.3 chaperone complex. Mol Cell Biol 31:4107-18 (2011). WB, ICC/IF, IP ; Human . PubMed: 21807893
- Elsaesser SJ & Allis CD HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Cold Spring Harb Symp Quant Biol 75:27-34 (2010). WB . PubMed: 21047901
