Anti-Calcineurin A抗体[EP1669Y] (ab52761)
![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.cn/ps/products/52/ab52761/Images/ab52761-450125-rabbit-monoclonal-ep1669y-to-calcineurin-a-western-blot-hela-lysates.jpg "Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)")
Key features and details
- Rabbit monoclonal [EP1669Y] to Calcineurin A
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Calcineurin A抗体[EP1669Y]
参阅全部 Calcineurin A 一抗 -
描述
兔单克隆抗体[EP1669Y] to Calcineurin A -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Hamster
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, SH-SY5Y and HAP1 cell lysates. Flow Cyt (intra): HeLa cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1669Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52761于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | (3) |
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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| 说明 |
|---|
|
Flow Cyt (Intra)
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
靶标
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功能
Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. -
序列相似性
Belongs to the PPP phosphatase family. PP-2B subfamily. -
细胞定位
Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle. - Information by UniProt
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数据库链接
- Entrez Gene: 5530 Human
- Entrez Gene: 19055 Mouse
- Entrez Gene: 24674 Rat
- Omim: 114105 Human
- SwissProt: Q08209 Human
- SwissProt: P63328 Mouse
- SwissProt: P63329 Rat
- Unigene: 435512 Human
see all -
别名
- Alpha isoform formerly PPP2B antibody
- Calcineurin A alpha antibody
- Calcineurin A1 antibody
see all
图片
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Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)All lanes : Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PPP3CA knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 59 kDa
Observed band size: 59 kDaLanes 1- 2: Merged signal (red and green). Green - ab52761 observed at 59 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab52761 was shown to react with Calcineurin A in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265130 (knockout cell lysate ab257181) was used. Wild-type HeLa and PPP3CA knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52761 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.cn/ps/products/52/ab52761/Images/ab52761-65191-anti-calcineurin-a-antibody-ep1669y-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Overlay histogram showing HeLa cells stained with ab52761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52761, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.cn/ps/products/52/ab52761/Images/ab52761-266696-anti-calcineurin-a-antibody-ep1669y-western-blot.jpg)
Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: SH-SY5Y cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab52761 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52761 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab52761 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)](https://www.abcam.cn/ps/products/52/ab52761/Images/ab52761-65192-anti-calcineurin-a-antibody-ep1669y-western-blot.jpg)
Western blot - Anti-Calcineurin A antibody [EP1669Y] (ab52761)Anti-Calcineurin A antibody [EP1669Y] (ab52761) at 1/10000 dilution + Hela cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 59 kDa
Observed band size: 59 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab52761 被引用在 10 文献中.
- Li MD et al. DHHC2 regulates fear memory formation, LTP, and AKAP150 signaling in the hippocampus. iScience 26:107561 (2023). PubMed: 37664599
- Zhang J et al. RCAN1.4 attenuates renal fibrosis through inhibiting calcineurin-mediated nuclear translocation of NFAT2. Cell Death Discov 7:317 (2021). PubMed: 34707090
- Xu Y et al. GDF-11 Protects the Traumatically Injured Spinal Cord by Suppressing Pyroptosis and Necroptosis via TFE3-Mediated Autophagy Augmentation. Oxid Med Cell Longev 2021:8186877 (2021). PubMed: 34712387
- Yang X et al. The Protective Effect of Qishen Granule on Heart Failure after Myocardial Infarction through Regulation of Calcium Homeostasis. Evid Based Complement Alternat Med 2020:1868974 (2020). PubMed: 33149749
- Liu Y et al. C1222C Deletion in Exon 8 of ABL1 Is Involved in Carcinogenesis and Cell Cycle Control of Colorectal Cancer Through IRS1/PI3K/Akt Pathway. Front Oncol 10:1385 (2020). PubMed: 32850446
- Shi W et al. Histone deacetylase 4 mediates high glucose-induced podocyte apoptosis via upregulation of calcineurin. Biochem Biophys Res Commun 533:1061-1068 (2020). PubMed: 33019979
- Fraseur JG & Kinzer-Ursem TL Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification. PLoS One 13:e0197120 (2018). PubMed: 29864125
- Leifheit-Nestler M et al. Impact of Altered Mineral Metabolism on Pathological Cardiac Remodeling in Elevated Fibroblast Growth Factor 23. Front Endocrinol (Lausanne) 9:333 (2018). PubMed: 29977226
- Chen Y et al. Lercanidipine attenuates angiotensin II-induced cardiomyocyte hypertrophy by blocking calcineurin-NFAT3 and CaMKII-HDAC4 signaling. Mol Med Rep 16:4545-4552 (2017). PubMed: 28849081
- Moreira-Gonçalves D et al. Intermittent cardiac overload results in adaptive hypertrophy and provides protection against left ventricular acute pressure overload insult. J Physiol 593:3885-97 (2015). PubMed: 26010517