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重组RabMAb

重组Anti-Cadherin 9抗体[EPR5113] (ab124803)

Reviews (1) Submit a question
Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
  • Anti-Cadherin 9 antibody [EPR5113] (ab124803)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5113] to Cadherin 9
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-Cadherin 9抗体[EPR5113]
    参阅全部 Cadherin 9 一抗

  • 描述

    兔单克隆抗体[EPR5113] to Cadherin 9

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WBmore details
    不适用于: ICC/IF,IHC-P or IP

  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Rat

  • 免疫原

    Synthetic peptide within Human Cadherin 9 aa 750 to the C-terminus (intracellular). The exact sequence is proprietary.

  • 阳性对照

    • 293T, U87-MG, Human testis, NIH/3T3 and Caco 2 lysates; Permeabilized 293T cells

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab124803于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
说明
Flow Cyt (Intra)
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 88 kDa).
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.

靶标

  • 功能

    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

  • 序列相似性

    Contains 5 cadherin domains.

  • 结构域

    Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.

  • 细胞定位

    Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • CADH9_HUMAN antibody
      • Cadherin 9 type 2 antibody
      • Cadherin-9 antibody
      • Cadherin9 antibody
      • CDH 9 antibody
      • CDH9 antibody
      • T1 cadherin antibody
      see all

    图片

    • Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Western blot - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      All lanes : Anti-Cadherin 9 antibody [EPR5113] (ab124803) at 1/1000 dilution

      Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
      Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 88 kDa
      Observed band size: 110 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Flow Cytometry (Intracellular) - Anti-Cadherin 9 antibody [EPR5113] (ab124803)

      Intracellular flow cytometric analysis of permeabilized 293T cells using ab124803 at 1/100 dilution (red) or a rabbit IgG (negative) (green).

       

    • OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      OI-RD Scanning - Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-Cadherin 9 antibody [EPR5113] (ab124803)
      Anti-Cadherin 9 antibody [EPR5113] (ab124803)

    文献 (0)

    发表研究结果有使用 ab124803？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab124803 尚未被引用在任何文献中。

    客户评价及客户问答

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cadherin 9 antibody [EPR5113]

     Inconclusive
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (ES Cells and Neural cells)
    Permeabilization
    Yes - 0.4%-1% Triton
    Specification
    ES Cells and Neural cells
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Maneeshi Prasad

    Verified customer

    提交于 Jul 21 2016

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