重组Anti-CACNA2D2抗体[EPR12343] (ab173293)
![Western blot - Anti-CACNA2D2 antibody [EPR12343] (ab173293)](https://www.abcam.cn/ps/products/173/ab173293/Images/ab173293-269775-anti-cacna2d2-antibody-epr12343-western-blot.jpg "Western blot - Anti-CACNA2D2 antibody [EPR12343] (ab173293)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12343] to CACNA2D2
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-CACNA2D2抗体[EPR12343]
参阅全部 CACNA2D2 一抗 -
描述
兔单克隆抗体[EPR12343] to CACNA2D2 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Mouse, Human
预测可用于: Rat
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免疫原
Recombinant fragment within Human CACNA2D2 aa 50-200. The exact sequence is proprietary.
Database link: Q9NY47 -
阳性对照
- Fetal lung, fetal brain and Human cerebellum lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12343 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173293于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/10 - 1/100.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 130 kDa.
|
| 说明 |
|---|
|
IP
1/10 - 1/100. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 130 kDa. |
靶标
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功能
The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G). Overexpression induces apoptosis. -
组织特异性
Predominantly present in cerebellar cortex. Present in various lung tumor cell lines, while it is absent in normal lung (at protein level). Highly expressed in heart, lung, testis, pancreas and skeletal muscle. Also expressed in kidney, liver, placenta and brain. -
序列相似性
Belongs to the calcium channel subunit alpha-2/delta family.
Contains 1 cache domain.
Contains 1 VWFA domain. -
结构域
The MIDAS-like motif in the VWFA domain binds divalent metal cations and is required to promote trafficking of the alpha-1 (CACNA1) subunit to the plasma membrane by an integrin-like switch. -
翻译后修饰
May be proteolytically processed into subunits alpha-2-2 and delta-2 that are disulfide-linked. It is however unclear whether such cleavage really takes place in vivo and has a functional role. -
细胞定位
Membrane. Colocalizes with CACNA1A in lipid raft fractions. - Information by UniProt
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数据库链接
- Entrez Gene: 9254 Human
- Entrez Gene: 56808 Mouse
- Entrez Gene: 300992 Rat
- Omim: 607082 Human
- SwissProt: Q9NY47 Human
- SwissProt: Q6PHS9 Mouse
- SwissProt: Q8CFG6 Rat
- Unigene: 476273 Human
see all -
别名
- Alpha 2 delta calcium channel subunit antibody
- CA2D2_HUMAN antibody
- CACNA2D antibody
see all
图片
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Western blot - Anti-CACNA2D2 antibody [EPR12343] (ab173293)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CACNA2D2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human brain tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab173293 observed at 160 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab173293 was shown to specifically react with CACNA2D2 when CACNA2D2 knockout samples were used. Wild-type and CACNA2D2 knockout samples were subjected to SDS-PAGE. ab173293 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-CACNA2D2 antibody [EPR12343] (ab173293)](https://www.abcam.cn/ps/products/173/ab173293/Images/ab173293-196696-anti-cacna2d2-antibody-epr12343-western-blot.jpg)
Western blot - Anti-CACNA2D2 antibody [EPR12343] (ab173293)All lanes : Anti-CACNA2D2 antibody [EPR12343] (ab173293) at 1/1000 dilution
Lane 1 : Fetal lung lysate
Lane 2 : Fetal brain lysate
Lane 3 : Human cerebellum lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 130 kDa -
![Immunoprecipitation - Anti-CACNA2D2 antibody [EPR12343] (ab173293)](https://www.abcam.cn/ps/products/173/ab173293/Images/ab173293-196695-anti-cacna2d2-antibody-epr12343-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CACNA2D2 antibody [EPR12343] (ab173293)Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173293.
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![Anti-CACNA2D2 antibody [EPR12343] (ab173293)](https://www.abcam.cn/ps/products/173/ab173293/Images/ab173293-4-benefits-of-recombinant-antibodies.png)
Anti-CACNA2D2 antibody [EPR12343] (ab173293)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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