Anti-CACNA1G抗体[N178A/9] (ab134269)
Key features and details
- Mouse monoclonal [N178A/9] to CACNA1G
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-CACNA1G抗体[N178A/9]
参阅全部 CACNA1G 一抗 -
描述
小鼠单克隆抗体[N178A/9] to CACNA1G -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Fusion protein corresponding to Mouse CACNA1G aa 2050-2200 (C terminal).
Database link: Q9WUT2 -
阳性对照
- WB: Rat brain membrane tissue lysate. ICC/IF: SK-N-BE cells.
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常规说明
The clone number has been updated from S178A-9 to N178A/9, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
N178A/9 -
同种型
IgG1
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134269于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 262 kDa.
1µg/ml of ab134269 is sufficient for detection of CACNA1G in 20µg of Rat brain membrane lysate. |
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ICC/IF |
1/100.
|
说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 262 kDa. 1µg/ml of ab134269 is sufficient for detection of CACNA1G in 20µg of Rat brain membrane lysate. |
ICC/IF
1/100. |
靶标
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功能
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1G gives rise to T-type calcium currents. T-type calcium channels belong to the "low-voltage activated (LVA)" group and are strongly blocked by mibefradil. A particularity of this type of channel is an opening at quite negative potentials and a voltage-dependent inactivation. T-type channels serve pacemaking functions in both central neurons and cardiac nodal cells and support calcium signaling in secretory cells and vascular smooth muscle. They may also be involved in the modulation of firing patterns of neurons which is important for information processing as well as in cell growth processes. -
组织特异性
Highly expressed in brain, in particular in the amygdala, subthalamic nuclei, cerebellum and thalamus. Moderate expression in heart; low expression in placenta, kidney and lung. Also expressed in colon and bone marrow and in tumoral cells to a lesser extent. Highly expressed in fetal brain, but also in peripheral fetal tissues as heart, kidney and lung, suggesting a developmentally regulated expression. -
疾病相关
Spinocerebellar ataxia 42 -
序列相似性
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1G subfamily. -
发展阶段
Isoform 4, isoform 7, isoform 18, isoform 19, isoform 24, isoform 25 and isoform 31 are adult-specific. Isoform 8, isoform 14, isoform 15, isoform 16, isoform 17, isoform 20, isoform 21, isoform 22, isoform 23, isoform 26, isoform 27, isoform 28, isoform 29 and isoform 30 are fetal-specific. Isoform 1, isoform 2, isoform 6, isoform 9, isoform 11, isoform 12 and isoform 13 are expressed in both fetus and adult. -
结构域
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
The linker region between repeat III and IV probably plays a role in the inactivation of the channel. The C-terminal part may be implicated in the anchoring of the protein to the membrane by interfering with or restricting its lateral diffusion. -
翻译后修饰
In response to raising of intracellular calcium, the T-type channels are activated by CaM-kinase II. -
细胞定位
Cell membrane. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 8913 Human
- Entrez Gene: 29717 Rat
- Omim: 604065 Human
- SwissProt: O43497 Human
- SwissProt: O54898 Rat
- Unigene: 591169 Human
- Unigene: 86960 Rat
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别名
- CAC1G_HUMAN antibody
- Cacna1g antibody
- calcium channel voltage dependent alpha 1G subunit antibody
see all
图片
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Anti-CACNA1G antibody [N178A/9] (ab134269) at 1/1000 dilution + Rat brain membrane tissue lysate
Secondary
Goat anti-mouse IgG HRP
Developed using the ECL technique.
Predicted band size: 262 kDa -
4% formaldehyde-fixed SK-N-BE cells stained for CACNA1G (green) using ab134269 at 1/100 dilution in ICC/IF.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.
A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134269. (D) Composite.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab134269 被引用在 4 文献中.
- Chen J et al. MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression. Environ Toxicol 39:357-366 (2024). PubMed: 37755144
- Koga S et al. PTPN3 is a potential target for a new cancer immunotherapy that has a dual effect of T cell activation and direct cancer inhibition in lung neuroendocrine tumor. Transl Oncol 14:101152 (2021). PubMed: 34134073
- Kim JW et al. Epigenetically Upregulated T-Type Calcium Channels Contribute to Abnormal Proliferation of Embryonic Neural Progenitor Cells Exposed to Valproic Acid. Biomol Ther (Seoul) 28:389-396 (2020). PubMed: 32319264
- Kim JW et al. T-Type Calcium Channels Are Required to Maintain Viability of Neural Progenitor Cells. Biomol Ther (Seoul) 26:439-445 (2018). PubMed: 29463073