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重组RabMAb

重组Anti-CAB39/MO25抗体[EP1680Y] (ab51132)

Submit a review Submit a question References (8)
Western blot - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
  • Flow Cytometry (Intracellular) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
  • Immunocytochemistry/ Immunofluorescence - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
  • Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1680Y] to CAB39/MO25
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-CAB39/MO25抗体[EP1680Y]
    参阅全部 CAB39/MO25 一抗

  • 描述

    兔单克隆抗体[EP1680Y] to CAB39/MO25

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human CAB39/MO25 (C terminal). The exact sequence is proprietary.

  • 阳性对照

    • HeLa cell lysate, human brain tissue, or HeLa cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab51132于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/20000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

We strongly recommend that customers perform an antigen retireval step.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
说明
WB
1/20000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

We strongly recommend that customers perform an antigen retireval step.
ICC/IF
1/100 - 1/250.
Flow Cyt (Intra)
1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

图片

  • Western blot - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Western blot - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Anti-CAB39/MO25 antibody [EP1680Y] (ab51132) at 1/20000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 35 kDa why is the actual band size different from the predicted?

  • Flow Cytometry (Intracellular) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Flow Cytometry (Intracellular) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)

    Overlay histogram showing HeLa cells stained with ab51132 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51132, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)

    Ab51132 (1:100) staining human CAB39/MO25 in human brain tissue by immunohistochemistry using paraffin embedded tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Immunocytochemistry/ Immunofluorescence - Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Immunofluorescent staining of HeLa cells using ab51132 (1:100).
  • Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)
    Anti-CAB39/MO25 antibody [EP1680Y] (ab51132)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (8)

发表研究结果有使用 ab51132？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab51132 被引用在 8 文献中.

  • Chen L  et al. RBM4 dictates ESCC cell fate switch from cellular senescence to glutamine-addiction survival through inhibiting LKB1-AMPK-axis. Signal Transduct Target Ther 8:159 (2023). PubMed: 37080995
  • Liu Y  et al. 6-Gingerol Ameliorates Hepatic Steatosis, Inflammation and Oxidative Stress in High-Fat Diet-Fed Mice through Activating LKB1/AMPK Signaling. Int J Mol Sci 24:N/A (2023). PubMed: 37047258
  • Zhang S  et al. Metformin-Induced MicroRNA-34a-3p Downregulation Alleviates Senescence in Human Dental Pulp Stem Cells by Targeting CAB39 through the AMPK/mTOR Signaling Pathway. Stem Cells Int 2021:6616240 (2021). PubMed: 33505470
  • Liang Y  et al. MiR-107 confers chemoresistance to colorectal cancer by targeting calcium-binding protein 39. Br J Cancer 122:705-714 (2020). PubMed: 31919406
  • Li J  et al. miR-451 Silencing Inhibited Doxorubicin Exposure-Induced Cardiotoxicity in Mice. Biomed Res Int 2019:1528278 (2019). PubMed: 31355248
  • Zeng J  et al. PrLZ increases prostate cancer docetaxel resistance by inhibiting LKB1/AMPK-mediated autophagy. Theranostics 8:109-123 (2018). PubMed: 29290796
  • Behunin SM  et al. Liver Kinase B1 complex acts as a novel modifier of myofilament function and localizes to the Z-disk in cardiac myocytes. Arch Biochem Biophys 601:32-41 (2016). PubMed: 26971467
  • Shields BB  et al. A genome-scale screen reveals context-dependent ovarian cancer sensitivity to miRNA overexpression. Mol Syst Biol 11:842 (2015). PubMed: 26655797

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