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Cardiovascular Blood Blood Cell Antigens

Anti-CA19-9抗体[121SLE] (ab3982)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (13)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)

    Key features and details

    • Mouse monoclonal [121SLE] to CA19-9
    • Suitable for: IHC-P, ELISA, Flow Cyt
    • Reacts with: Human
    • Isotype: IgM

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    概述

    • 产品名称

      Anti-CA19-9抗体[121SLE]
      参阅全部 CA19-9 一抗
    • 描述

      小鼠单克隆抗体[121SLE] to CA19-9
    • 宿主

      Mouse
    • 特异性

      The antibody recognizes Sialyl Lewis a containing glycolipids.
    • 经测试应用

      适用于: IHC-P, ELISA, Flow Cytmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Tissue, cells or virus corresponding to CA19-9. Immunoprecipitate obtained after immunodiffusion of MAb 19-9 and mucins isolated from an ovarian cyst of a 0Le(a+b-) patient.

    • 阳性对照

      • IHC-P: Colorectal adenocarcinoma.
    • 常规说明

      This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C. Do Not Freeze.
    • 存储溶液

      pH: 8.0
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Proprietary Purification
    • 纯化说明

      Purified from TCS. Purified by precipitation and chromatography. Purity >95% by SDS-PAGE.
    • 克隆

      单克隆
    • 克隆编号

      121SLE
    • 同种型

      IgM
    • 研究领域

      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Cancer
      • Tumor biomarkers
      • Tumor antigens
      • Cardiovascular
      • Blood
      • Blood Cell Antigens
      • RBC Antigens
      • Blood Group Antigens

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
      • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
      • Goat Anti-Mouse IgM H&L (ab9167)
      • Rabbit Anti-Mouse IgM mu chain (ab9175)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3982于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P
    1/100.
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 22490524

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    说明
    IHC-P
    1/100.
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration. PubMed: 22490524

    ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

    靶标

    • 别名

      • CA19.9 antibody
      • Ovarian tumor antigen antibody
      • Pancreatic tumor antigen antibody
      • Sialyl Lewis a antibody

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA19-9 antibody [121SLE] (ab3982)

      ab3982 staining CA19-9 in human colorectal adenocarcinoma by IHC-P (Formalin/Paraformaldehye fixed paraffin embedded tissue sections).

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (13)

    发表研究结果有使用 ab3982?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3982 被引用在 13 文献中.

    • Starzonek S  et al. Systematic analysis of the human tumor cell binding to human vs. murine E- and P-selectin under static vs. dynamic conditions. Glycobiology 30:695-709 (2020). PubMed: 32103235
    • Hu H  et al. MicoRNA-301a Promotes Pancreatic cancer Invasion and Metastasis through the JAK/STAT3 Signaling Pathway by Targeting SOCS5. Carcinogenesis N/A:N/A (2019). PubMed: 31233116
    • Purohit S  et al. Multiplex glycan bead array for high throughput and high content analyses of glycan binding proteins. Nat Commun 9:258 (2018). PubMed: 29343722
    • Tang H  et al. Glycans related to the CA19-9 antigen are elevated in distinct subsets of pancreatic cancers and improve diagnostic accuracy over CA19-9. Cell Mol Gastroenterol Hepatol 2:201-221.e15 (2016). PubMed: 26998508
    • Gomez DL  et al. Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS One 10:e0133862 (2015). IHC . PubMed: 26288179
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    Western blot abreview for Anti-CA19-9 antibody [121SLE]

    Below Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    40 µg
    Specification
    Melanoma cell line
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Oct 19 2015

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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