AB168345

Anti-C9 抗体 [EPR11232]

Anti-C9 antibody [EPR11232]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal C9 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications.

查看别名

Complement component C9, C9

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody [EPR11232] (AB168345)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling C9 with ab168345 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-C9 antibody [EPR11232] (AB168345)

Immunoprecipitation : Western blot analysis on immunoprecipitation pellet from Human plasma cell lysate using ab168345 at a 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-C9 antibody [EPR11232] (ab168345) at 1/10 dilution

All lanes:

Human plasma cell lysate

Predicted band size: 63 kDa

false

Unknown

Western blot - Anti-C9 antibody [EPR11232] (AB168345)

All lanes:

Anti-ZNF239 antibody (<a href='/products/unavailable/znf239-antibody-ab118345'>ab118345</a>) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

Human angioneoplasm lysate at 10 µg

Lane 3:

Human placenta lysate at 10 µg

Lane 4:

Human serum lysate at 10 µg

Lane 5:

Human plasma lysate at 10 µg

Predicted band size: 63 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-C9 antibody [EPR11232] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11232

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C9 also known as complement component 9 is a protein that plays an essential mechanical role in the complement system. It typically associates in the formation of the membrane attack complex (MAC). The C9 protein weighing around 71 kDa expresses in the liver and is secreted into the blood plasma. Alternative names like 'Cloud 9 capture' and 'C9 tag' often refer to research tools or motifs derived from the C9 protein rather than the protein itself. Researchers frequently study C9 in mouse models where its physiological roles can be explored.

Biological function summary

C9 participates in defending against pathogens. It joins other complement components like C5b C6 C7 and C8 to form the MAC complex which facilitates cell lysis by forming pores in the membrane of target cells. This action directly results in microbial cell destruction serving a critical immune function. C9 is not only part of the MAC complex but also holds significance due to its unique function as the last step in the formation of the pore influencing the efficacy of pathogen elimination.

Pathways

C9 functions primarily within the complement cascade which includes the classical and alternative pathways. Its role is in the terminal pathway segment that ultimately leads to the formation of the MAC and follows the activation of C5 which cleaves to produce C5b. This connection extends to proteins like C3 which is upstream in the cascade and significantly impacts the cascade's activation highlighting C9's function as an integral terminal component in these pathways.

Mutations or deficits in C9 relate to an increased susceptibility to infections particularly those caused by Neisseria species. This association results directly from its important role in the MAC formation. Additionally deficiency of C9 links to autoimmune disorders where the immune response may be improperly regulated due to compromised MAC formation. Other complement components like C6 and C8 may also relate to these diseases due to their collaboration with C9 in forming the MAC illustrating the interconnectedness of these proteins within the immune response framework.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Pore-forming component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 22832194, PubMed : 26841837, PubMed : 26841934, PubMed : 27052168, PubMed : 30552328, PubMed : 6177822, PubMed : 9212048, PubMed : 9634479). The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways (PubMed : 39914456, PubMed : 39814882, PubMed : 9212048, PubMed : 9634479). The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 9212048, PubMed : 9634479). Constitutes the pore-forming subunit of the MAC complex : during MAC assembly, C9 associates with the C5b8 intermediate complex, and polymerizes to complete the pore (PubMed : 26841934, PubMed : 30111885, PubMed : 30552328, PubMed : 34752492, PubMed : 4055801, PubMed : 6177822).

See full target information C9

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 22:12 PubMed40229672

2025

Proteomic profiles screening identified novel exosomal protein biomarkers for diagnosis of lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyan Feng,Ying Lin,Ling Zhang,Weiming Hu

Renal failure 47:2440528 PubMed39806768

2025

IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jieling Liang,Yangxiao Huang,Daping Peng,Yali Xie,Yifei Liu,Xiuxia Lu,Junfa Xu

Acta biochimica et biophysica Sinica 54:1694-1707 PubMed35929594

2022

Comprehensive analysis of complement-associated molecular features in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Run Huang,Guiqi Zhu,Xiutao Fu,Weiren Liu,Chenyang Tao,Jun Gao,Weifeng Qu,Yuan Fang,Xifei Jiang,Zhenbin Ding,Jian Zhou,Yinghong Shi,Jia Fan,Zheng Tang

Medical science monitor basic research 25:33-44 PubMed30713336

2019

Autophagy and Oncosis/Necroptosis Are Enhanced in Cardiomyocytes from Heart Failure Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanni Corsetti,Carol Chen-Scarabelli,Claudia Romano,Evasio Pasini,Francesco S Dioguardi,Francesco Onorati,Richard Knight,Hemang Patel,Louis Saravolatz,Giuseppe Faggian,Tiziano M Scarabelli

View all publications

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Anti-C9 抗体 [EPR11232]