AB181031

重组Anti-C8orf30A抗体[EPR13203-28]

Anti-C8orf30A antibody [EPR13203-28]

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal C8orf30A antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

查看别名

C8orf30A, C8orf30B, FAM203A, FAM203B, HGH1, Protein HGH1 homolog

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human breast carcinoma tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human endometrium tissue labeling C8orf30A with ab181031 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling C8orf30A with ab181031 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Supplier Data

Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (+) or a negative control (-) immunoprecipitated using ab181031 at 1/40 dilution.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-C8orf30A antibody [EPR13203-28] (ab181031)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-C8orf30A antibody [EPR13203-28] (AB181031)

All lanes:

Western blot - Anti-C8orf30A antibody [EPR13203-28] (ab181031) at 1/5000 dilution

Lane 1:

Human fetal liver tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

true

不同偶联物与剂型 (1)

Carrier free

Anti-C8orf30A antibody [EPR13203-28] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR13203-28

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The C8orf30A protein also called Chromosome 8 open reading frame 30A has a molecular mass of about 12 kDa. This protein's function involves cellular processes related to energy metabolism and plays an unclear yet significant mechanical role in the cells. Expression of C8orf30A occurs in tissues that display high metabolic activity such as liver and muscle tissues. Current research suggests its activity might impact the efficiency of cellular energy utilization.

Biological function summary

C8orf30A contributes to the regulation of mitochondrial function and energy metabolism. It forms a part of a larger protein complex that participates in the cellular energy management. The presence of this protein influences the mitochondrial respiratory chain affecting the production of ATP—a critical energy currency of the cell. The modulation of energy dynamics by C8orf30A highlights its importance in maintaining cellular energy homeostasis.

Pathways

C8orf30A involvement reflects in the regulation of oxidative phosphorylation and glycolytic pathways. This protein interacts closely with enzymes and other proteins within these pathways to modulate energy production and balance. It associates with proteins like ATP synthase and complexes involved in electron transport reflecting an essential integrative role within metabolic pathways to harness energy efficiently.

Changes in C8orf30A levels connect to metabolic disorders including diabetes and metabolic syndrome. The protein's regulation influences factors related to these diseases impacting insulin sensitivity and energy utilization in cells. C8orf30A interacts with proteins such as glucose transporters and alters their function emphasizing its role in disease progression and providing potential targets for therapeutic intervention.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

See full target information HGH1

style

left-column

文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 28: PubMed37326104

2023

Construction of ceRNA regulatory networks for osteoporosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao Chen,Hailong Wang,Xu Liu,Long Li,Yiliyaer Abudusimu,Yilihamu Tuoheti

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-C8orf30A抗体[EPR13203-28]