Anti-C6抗体(ab74638)
Key features and details
- Rabbit polyclonal to C6
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C6抗体
参阅全部 C6 一抗 -
描述
兔多克隆抗体to C6 -
宿主
Rabbit -
特异性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow, Pig
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免疫原
Synthetic peptide corresponding to Human C6 aa 200-300 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab74637) -
阳性对照
- This antibody gave a positive signal in Human Plasma (Total Protein)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Matched antibody pairs
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab74638于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
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| ICC/IF |
Use a concentration of 5 µg/ml.
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| 说明 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). |
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ICC/IF
Use a concentration of 5 µg/ml. |
靶标
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功能
Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. -
疾病相关
Defects in C6 are the cause of complement component 6 deficiency (C6D) [MIM:612446]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. -
序列相似性
Belongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 2 Kazal-like domains.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 2 Sushi (CCP/SCR) domains.
Contains 3 TSP type-1 domains. -
翻译后修饰
All cysteine residues are assumed to be cross-linked to one another. Individual modules containing an even number of conserved cysteine residues are supposed to have disulfide linkages only within the same module. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 729 Human
- Entrez Gene: 12274 Mouse
- Omim: 217050 Human
- SwissProt: P13671 Human
- Unigene: 481992 Human
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别名
- AW111623 antibody
- C6 antibody
- CO6_HUMAN antibody
see all
图片
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Western blot - Anti-C6 antibody (ab74638)All lanes : Anti-C6 antibody (ab74638) at 1 µg/ml
Lane 1 : Human Plasma Total Protein Lysate at 2 µg
Lane 2 : Human Plasma Total Protein Lysate at 5 µg
Lane 3 : Human Plasma Total Protein Lysate at 10 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
C6 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. -
Immunocytochemistry/ Immunofluorescence - Anti-C6 antibody (ab74638)ICC/IF image of ab74638 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74638, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa cells at 5µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab74638 尚未被引用在任何文献中。