JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB241985

Anti-C5orf25抗体

Anti-C5orf25 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal C5orf25 antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SIMC1 aa 1-100.

查看别名

C5orf25, PLEIAD, SIMC1, SUMO-interacting motif-containing protein 1, Platform element for inhibition of autolytic degradation

1 Images
Immunoprecipitation - Anti-C5orf25 antibody (AB241985)
  • IP

Supplier Data

Immunoprecipitation - Anti-C5orf25 antibody (AB241985)

C5orf25 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241985 at 8 μL per reaction. Western blot was performed from the immunoprecipitate using ab241985 at 1/1000 dilution.

Lane 1 : ab241985 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-C5orf25 antibody (ab241985)

Predicted band size: 97 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IP

applications

免疫原

Synthetic Peptide within Human SIMC1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NDZ2

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>8 - 15 μl/mg lysate</p>" } } }
style
left-column
style
left-column

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
Antibody was affinity purified using an epitope specific to C5orf25 immobilized on solid support.
存储溶液
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C5orf25 also known as Chromosome 5 open reading frame 25 is a protein with a molecular weight of approximately 17 kDa. This protein shows expression in various tissues with notable presence in the brain and heart. C5orf25 has no definitive enzymatic function identified yet but studies suggest it might play roles in cellular processes through potential interactions with other cellular components. Understanding the mechanical role of C5orf25 remains an active area of research.
Biological function summary

This protein contributes to intracellular functions although not clearly defined. C5orf25 may partake in cellular signaling or structural integrity within specific cells. It does not appear as part of any well-characterized protein complex currently suggesting that it might function independently or as part of transient interactions in cellular biology. This protein’s biological roles could influence how cells respond to stress or maintain their regular operations.

Pathways

Researchers have begun mapping its interactions to various cellular processes. Current findings suggest potential links to signal transduction pathways such as the MAPK signaling. In this context it might interact indirectly with proteins like ERK and MEK known for regulating cell growth and differentiation. Although these connections are still theoretical they form the basis for further investigations into how C5orf25 fits into broader cellular networks.

Researchers have explored links between C5orf25 and neurological conditions. Some studies propose that variations in this protein might associate with neurodevelopmental disorders alongside proteins like DISC1. Furthermore altered expression levels of C5orf25 in cardiac tissues have drawn attention to possible involvement in heart diseases. Understanding these associations could help develop biomarkers or novel therapeutic targets leveraging the relationship between C5orf25 and disease pathways.
style
left-column
style
left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

靶点信息

Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SLF2 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication.. Isoform 1. Inhibits the protease activity of CAPN3.. Isoform 5. Inhibits the protease activity of CAPN3.
See full target information SIMC1
style
left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed36373674

2022

The Nse5/6-like SIMC1-SLF2 complex localizes SMC5/6 to viral replication centers.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Oravcová,Minghua Nie,Nicola Zilio,Shintaro Maeda,Yasaman Jami-Alahmadi,Eros Lazzerini-Denchi,James A Wohlschlegel,Helle D Ulrich,Takanori Otomo,Michael N Boddy

Frontiers in cell and developmental biology 7:351 PubMed31921861

2019

Role of TGF-β/Smad Pathway in the Transcription of Pancreas-Specific Genes During Beta Cell Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhua Gao,Ranxi Zhang,Shanshan Dai,Xue Zhang,Xiangchen Li,Chunyu Bai
View all publications
style
left-column
style
left-column
style
right-column

搭配使用产品

Primary Antibodies

AB234426

Anti-SV40 T-antigen antibody [EPR22694-148]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-SV40 T-antigen antibody [EPR22694-148] (AB234426)

  • 0
  • 0
Primary Antibodies

AB124673

Anti-Werner's syndrome helicase WRN antibody [EPR6392]

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (AB124673)

  • 4
  • 1
Primary Antibodies

AB241612

Anti-ZC3H11A antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZC3H11A antibody (AB241612)

  • 0
  • 0
Primary Antibodies

AB241578

Anti-UTP18 antibody

Immunoprecipitation - Anti-UTP18 antibody (AB241578)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com