Anti-C5a-R抗体(ab59390)
Key features and details
- Rabbit polyclonal to C5a-R
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C5a-R抗体
参阅全部 C5a-R 一抗 -
描述
兔多克隆抗体to C5a-R -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide corresponding to Human C5a-R. non-phosphopeptide derived from human C5a-R around the phosphorylation site of serine 338.
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阳性对照
- WB: Extracts from HeLa cells treated with PMA (125ng/ml, 30mins).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab59390于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa).
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| IHC-P |
Use a concentration of 1 µg/ml.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa). |
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IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Sulfation plays a critical role in the association of the receptor with C5a, but no significant role in the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 728 Human
- Entrez Gene: 12273 Mouse
- Omim: 113995 Human
- SwissProt: P21730 Human
- SwissProt: P30993 Mouse
- SwissProt: Q3TZ86 Mouse
- Unigene: 2161 Human
- Unigene: 247623 Mouse
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别名
- ANAPHYLATOXIN RECEPTOR antibody
- C5a anaphylatoxin chemotactic receptor antibody
- C5a anaphylatoxin receptor antibody
see all
图片
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Western blot - Anti-C5a-R antibody (ab59390)All lanes : Anti-C5a-R antibody (ab59390) at 1/500 dilution
Lane 1 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins).
Lane 2 : Extracts from HeLa cells, treated
with PMA (125ng/ml, 30mins) plus peptide.
Predicted band size: 39 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a-R antibody (ab59390)ab59390 staining C5a-R in human lung.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab59390 被引用在 11 文献中.
- Li C et al. Xuanfei Baidu Decoction suppresses complement overactivation and ameliorates IgG immune complex-induced acute lung injury by inhibiting JAK2/STAT3/SOCS3 and NF-κB signaling pathway. Phytomedicine 109:154551 (2023). PubMed: 36610119
- Beach C et al. Improving radiotherapy in immunosuppressive microenvironments by targeting complement receptor C5aR1. J Clin Invest 133:N/A (2023). PubMed: 37824211
- Shibata R et al. Administration of C5a Receptor Antagonist Improves the Efficacy of Human Induced Pluripotent Stem Cell-Derived Neural Stem/Progenitor Cell Transplantation in the Acute Phase of Spinal Cord Injury. J Neurotrauma 39:667-682 (2022). PubMed: 35196890
- Kim EN et al. CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm. PLoS One 16:e0245361 (2021). PubMed: 34428207
- Deng S et al. Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion. PLoS Negl Trop Dis 15:e0009508 (2021). PubMed: 34237073