重组Anti-C4d抗体[SP91] - C-terminal (ab183311)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP91] to C4d - C-terminal
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-C4d抗体[SP91] - C-terminal
参阅全部 C4d 一抗 -
描述
兔单克隆抗体[SP91] to C4d - C-terminal -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- IHC-P: Human tonsil tissue.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP91 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
- Anti-C4d antibody [SP91] - BSA and Azide free (ab236231)
- Alexa Fluor® 488 Anti-C4d antibody [SP91] - C-terminal (ab320605)
- Alexa Fluor® 647 Anti-C4d antibody [SP91] - C-terminal (ab320606)
- Alexa Fluor® 555 Anti-C4d antibody [SP91] - C-terminal (ab320607)
- Alexa Fluor® 594 Anti-C4d antibody [SP91] - C-terminal (ab320608)
- PE Anti-C4d antibody [SP91] - C-terminal (ab320609)
- APC Anti-C4d antibody [SP91] - C-terminal (ab320610)
- Alexa Fluor® 750 Anti-C4d antibody [SP91] - C-terminal (ab321266)
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183311于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
1/100.
Perform Antigen Retrieval by boiling tissue sections in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
说明 |
---|
IHC-P
1/100. Perform Antigen Retrieval by boiling tissue sections in 1mM EDTA, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. |
靶标
-
功能
C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway.
Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
疾病相关
Defects in C4A are the cause of complement component 4A deficiency (C4AD) [MIM:120810]. A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis. -
序列相似性
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
翻译后修饰
Prior to secretion, the single-chain precursor is enzymatically cleaved to yield the non-identical chains (alpha, beta and gamma). During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase.
N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 100293534 Human
- Entrez Gene: 100507685 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
see all -
别名
- acidic C4 antibody
- Acidic complement C4 antibody
- basic C4 antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue. The section was incubated with ab183311 at 1:100 dilution for 30 mins at room temperature. Heat-mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. A secondary antibody-only negative control was also performed. Hematoxylin was used as a counterstain. Positive staining was observed in the germinal centre of human tonsil performed on a Leica Biosystems BOND® RX instrument.
-
Immunohistochemical analysis of Human tonsil tissue labeling C4d using ab183311 at a 1/100 dilution
-
Immunohistochemical analysis of Human tonsil tissue labeling C4d using ab183311 at a 1/100 dilution.
数据表及文件
-
SDS download
-
Datasheet download
文献 (2)
ab183311 被引用在 2 文献中.
- Jiao Y et al. Activation of complement C1q and C3 in glomeruli might accelerate the progression of diabetic nephropathy: Evidence from transcriptomic data and renal histopathology. J Diabetes Investig 13:839-849 (2022). PubMed: 34932275
- Deng M et al. Analyses of the clinical and immunological characteristics of patients with lupus erythematosus. Indian J Dermatol 67:205 (2022). PubMed: 36092230