重组Anti-C4BPA抗体[EPR14781(B)] - C-terminal (ab182140)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14781(B)] to C4BPA - C-terminal
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-C4BPA抗体[EPR14781(B)] - C-terminal
参阅全部 C4BPA 一抗 -
描述
兔单克隆抗体[EPR14781(B)] to C4BPA - C-terminal -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human plasma lysate. Human fetal liver lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR14781(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab182140于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
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IP |
1/60.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). |
IP
1/60. |
靶标
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功能
Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component. -
组织特异性
Chylomicrons in the plasma. -
序列相似性
Contains 8 Sushi (CCP/SCR) domains. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 722 Human
- Entrez Gene: 12269 Mouse
- Omim: 120830 Human
- SwissProt: P04003 Human
- SwissProt: P08607 Mouse
- Unigene: 1012 Human
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别名
- C4b-binding protein alpha chain antibody
- C4bp antibody
- C4bpa antibody
see all
图片
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Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/10000 dilution + Human plasma lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Anti-C4BPA antibody [EPR14781(B)] - C-terminal (ab182140) at 1/2000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 67 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Western blot analysis of C4BPA in Human plasma immunoprecipitated with ab182140 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody used at 1/1000 dilution.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab182140 被引用在 1 文献中.
- Tougan T et al. The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity. Malar J 19:155 (2020). PubMed: 32295584