重组Anti-C4抗体[EPR2990(2)] (ab173577)
![Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)](https://www.abcam.cn/ps/products/173/ab173577/Images/ab173577-197098-anti-c4-antibody-epr29902-western-blot.jpg "Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2990(2)] to C4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-C4抗体[EPR2990(2)]
参阅全部 C4 一抗 -
描述
兔单克隆抗体[EPR2990(2)] to C4 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human C4 aa 1200-1300 (Cysteine residue). The exact sequence is proprietary. Note: this sequence is identical in both C4 subunits A (P0C0L4) and B (P0C0L5).
Database link: P0C0L4 -
阳性对照
- WB: HepG2 cell lysate. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR2990(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab173577于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/20.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 193 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/20. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 193 kDa. |
|
ICC/IF
1/100 - 1/250. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 100293534 Human
- Entrez Gene: 720 Human
- Entrez Gene: 721 Human
- Omim: 120810 Human
- Omim: 120820 Human
- SwissProt: P0C0L4 Human
- SwissProt: P0C0L5 Human
- Unigene: 534847 Human
see all -
别名
- Acidic C4 antibody
- Acidic complement C4 antibody
- Basic C4 antibody
see all
图片
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Western blot - Anti-C4 antibody [EPR2990(2)] (ab173577)Anti-C4 antibody [EPR2990(2)] (ab173577) at 1/1000 dilution + HepG2 cell lysate at 10 µg
Predicted band size: 193 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-C4 antibody [EPR2990(2)] (ab173577)](https://www.abcam.cn/ps/products/173/ab173577/Images/ab173577-197099-anti-c4-antibody-epr29902-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C4 antibody [EPR2990(2)] (ab173577)Immunofluorescent staining of HepG2 cells labeling C4 with ab173577 at 1/100
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![Flow Cytometry (Intracellular) - Anti-C4 antibody [EPR2990(2)] (ab173577)](https://www.abcam.cn/ps/products/173/ab173577/Images/ab173577-280381-anti-c4-antibody-epr29902-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-C4 antibody [EPR2990(2)] (ab173577)Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling C4 (red) with ab173577 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Anti-C4 antibody [EPR2990(2)] (ab173577)](https://www.abcam.cn/ps/products/173/ab173577/Images/ab173577-4-benefits-of-recombinant-antibodies.png)
Anti-C4 antibody [EPR2990(2)] (ab173577)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab173577 被引用在 4 文献中.
- Sager REH et al. Trajectory of change in brain complement factors from neonatal to young adult humans. J Neurochem 157:479-493 (2021). PubMed: 33190236
- Purves-Tyson TD et al. Increased Macrophages and C1qA, C3, C4 Transcripts in the Midbrain of People With Schizophrenia. Front Immunol 11:2002 (2020). PubMed: 33133060
- Sellgren CM et al. Increased synapse elimination by microglia in schizophrenia patient-derived models of synaptic pruning. Nat Neurosci 22:374-385 (2019). PubMed: 30718903
- Sellgren CM et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry 22:170-177 (2017). WB, ICC/IF ; Human . PubMed: 27956744