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AB234631

Anti-C3b / iC3b抗体[1H8] - BSA and Azide free

Anti-C3b / iC3b antibody [1H8] - BSA and Azide free

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Mouse Monoclonal C3 antibody. Carrier free. Suitable for WB, ELISA and reacts with Human samples.

查看别名

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

1 Images
Western blot - Anti-C3b / iC3b antibody [1H8] - BSA and Azide free (AB234631)
  • WB

Lab

Western blot - Anti-C3b / iC3b antibody [1H8] - BSA and Azide free (AB234631)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before being incubated with ab231080 overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/20000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab231080).

All lanes:

Western blot - Anti-C3b / iC3b antibody [1H8] - BSA and Azide free (ab234631)

Lane 1:

human serum diluted 1/100 at 5 µL

Lane 2:

human plasma diluted 1/100 at 5 µL

Predicted band size: 187 kDa

Observed band size: 68 kDa

false

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

1H8

亚型

IgG2

轻链类型

kappa

不含载体蛋白

Yes

反应种属

Human

应用

ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

ab234631 is the carrier-free version of ab231080.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein G
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C3b protein also known as complement component 3b plays a significant role in the immune system aiding in the clearance of pathogens. It is a large glycoprotein with a mass of approximately 180 kDa. C3b is expressed mainly in the liver and released into the bloodstream as part of the complement system. Upon activation from its precursor C3 it becomes involved in opsonization enhancing the phagocytosis of pathogens by marking them for destruction by immune cells.
Biological function summary

C3b acts as a critical mediator in the immune system by forming complexes with other complement proteins. It is part of the alternative and classical pathways of the complement system. C3b can also convert itself into iC3b after cleavage by factor I and iC3b continues to facilitate phagocytosis but with reduced ability to promote further complement activation. This conversion ensures homeostasis and regulation preventing overactivation of the immune system.

Pathways

C3b participates in both the classical and alternative pathways of the complement system. It associates with proteins such as factor B and factor D to form the C3bBb complex in the alternative pathway acting as a C3 convertase that amplifies response. In the classical pathway C3b enhances the activity of C5 convertase facilitating the formation of the membrane attack complex. These pathways are vital in maintaining immune surveillance and response to infections.

Dysfunction or dysregulation of C3b activity links to autoimmune diseases and infectious diseases. For example atypical hemolytic uremic syndrome (aHUS) involves uncontrolled complement activation due to mutations affecting proteins like C3b. In such conditions C3b and related proteins such as factor H become therapeutic targets to modulate the complement system's overactivation. Studying the role of C3b in these diseases provides insight into potential interventions and novel treatments to manage complement-related pathologies.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).
See full target information C3
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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