重组Anti-C3a R抗体[hC3aRZ8] - BSA and Azide free (ab317633)

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling C3a R with ab317632 at 1/1000 (0.969 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Positive staining on human placenta.
The section was incubated with ab317632 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling C3a R with ab317632 at 1/1000 (0.969 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Positive staining on human ovarian cancer.
The section was incubated with ab317632 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling C3a R with ab317632 at 1/1000 (0.969 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Positive staining on human gastric cancer.
The section was incubated with ab317632 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a C3AR1 expression vector containing a his tag. (B) HEK-293T cells transfected with empty vector containing a his tag. tissue labeling C3a R with ab317632 at 1/1000 (0.969 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Positive staining on (A) HEK-293T cells transfected with a C3AR1 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.
The section was incubated with ab317632 for 30 mins at room temperature, followed by anti-mouse IgG2b antibody (ab190482) for 8 mins during the LeicaDS9800 kit staining procedure.
The immunostaining was performed on a Leica Biosystems BOND ® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling C3a R with ab317632 at 1/500 dilution (0.1 ug)/Right compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Mouse IgG (Alexa Fluor ® 488, ab150113) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with CD14 conjugated to Alexa Fluor ®647.

This data was developed using ab317632, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling C3a R with ab317632 at 1/500 dilution (0.1 ug)/Right compared with a Mouse monoclonal IgG / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Mouse IgG (Alexa Fluor ® 488, ab150113) at 1/5000 dilution was used as the secondary antibody.

Cells are co-stained with CD3 conjugated to Alexa Fluor ®647. The staining result showed a distinct CD3 positive population with no cross signal on C3a R positive cells.