AB11862

Anti-C3 抗体 [11H9]

Name: C3抗体[11H9] (ab11862)| Abcam中文官网
Brand: Abcam Limited
SKU: AB11862
Price: 5345 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-C3 antibody [11H9]

(4 Reviews)

(107 Publications)

Anti-C3 antibody [11H9] (ab11862) is a rat monoclonal antibody detecting C3/C3b in ICC/IF. Suitable for Mouse.

- Over 50 publications
- Trusted since 2004

查看别名

Complement C3, HSE-MSF, C3

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

ab11862 staining C3 in murine kidney cells by Immunocytochemistry/ Immunofluorescence.C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Glomerular staining pattern.

关键信息

宿主种属

Rat

克隆

Monoclonal

克隆号

11H9

亚型

IgG2a

不含载体蛋白

反应种属

Mouse

应用

ICC/IF

applications

免疫原

Cell preparation containing C3 protein. The exact immunogen used to generate this antibody is proprietary information.

P01027

特异性

This antibody recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg.The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linkedby disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to formiC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. Does not cross react with C4.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }

style

left-column

产品详情

What is this antibody validated in?
Anti-C3 antibody [11H9] (ab11862) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-C3 [11H9] (ab11862) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

0.2 µm filtered

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.

Biological function summary

Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.

Pathways

The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.

Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.

See full target information C3

style

left-column

文献 (107)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1446 PubMed41068254

2025

Cxcl9 macrophages recruit circulating Cxcr3+ plasmablasts into kidneys to promote pathogenesis of lupus nephritis mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Xinlong Bai,Cheng Zhou,Qing Ouyang,Yingjie Zhang,Xiao Zhang,Xumin Zheng,Chaofan Li,Wanjun Shen,Qinggang Li,Guangyan Cai,Xiangmei Chen,Ping Li,Xue-Yuan Bai

Nature 647:498-505 PubMed40931063

2025

Loss-of-function mutations in PLD4 lead to systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Qintao Wang,Honghao Zhu,Xiangwei Sun,Changming Zhang,Shuangyue Ma,Ying Jin,Jinjian Fu,Chenlu Liu,Jiahui Peng,Ruoran Wang,Lin Liu,Yi Zeng,Cheng Gong,Qing Zhou,Xiaomin Yu,Zhihong Liu

Haematologica : PubMed40820810

2025

KLF4 overexpression protects against complement-mediated endothelial injury in transplant-associated thrombotic microangiopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuhui Jiang,Jiaqian Qi,Tingting Pan,Zhenzhen Yao,Siyi Lu,Yifei Han,Depei Wu,Yue Han

IBRO neuroscience reports 19:300-306 PubMed40747365

2025

Reactive astrocyte-derived neurotoxicity is mitigated by vitronectin in traumatic brain injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Minori Yamashita,Nito Nakahira,Kei Hashimoto,Hirono Kobayashi,Mari Nakashima,Hiroko Ikeshima-Kataoka,Yasunori Miyamoto

ACS omega 10:28446-28465 PubMed40657111

2025

Drug Repurposing by Virtual Screening: Identification of New Already Approved ROCK Inhibitors as Promising Drugs to Target Neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Lucas Silva Franco,Daniel Alencar Rodrigues,Gabriela Joras Baumart,Bárbara da Silva Mascarenhas de Jesus,Flávia Carvalho Alcantara Gomes,Lídia Moreira Lima,Carlos Alberto Manssour Fraga,Pedro de Sena Murteira Pinheiro

Cell death discovery 11:320 PubMed40651955

2025

Inhibition of VEGFR-3 by SAR131675 decreases renal inflammation and lymphangiogenesis in the murine lupus nephritis model.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Wang,Wenjia Li,Ji-Hyun Yeom,Zhiheng Liu,Kyoung Min Kim,Kyung Pyo Kang

Journal of inflammation research 18:8579-8592 PubMed40620607

2025

Immune Cell Characteristics in a Gut-Kidney Axis-Induced Mouse Model of IgA Nephropathy: The Upregulated Dendritic Cells and Neutrophils.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqi Liu,Yuna Chen,Qijun Wan

BMC microbiology 25:412 PubMed40615777

2025

FabG moonlights as an extracellular adhesin mediates cytoadhesion of Streptococcus suis via interaction with plasminogen.

Applications

Unspecified application

Species

Unspecified reactive species

Genglin Guo,Yu Zhou,Pei Li,Quan Li,Yanfei Yu,Wei Zhang

Journal of neuroinflammation 22:69 PubMed40055743

2025

Intravitreal delivery of NMO-IgG causes primary retinal damage in the absence of optic nerve injury.

Applications

Unspecified application

Species

Unspecified reactive species

Biyue Chen,Huanfen Zhou,Mingming Sun,Wanqun Yang,Qianqian Li,Kaishu Yang,Honglu Song,Quangang Xu,Xintong Xu,Yuyu Li,Yanyan Yu,Shihui Wei,Tingjun Chen

mSystems 10:e0154024 PubMed40008883

2025

Dual RNA-seq reveals the complement protein C3-mediated host-pathogen interaction in the brain abscess caused by .

Applications

Unspecified application

Species

Unspecified reactive species

Qiyuan Jin,Yaxuan Zhai,Rui Qiang,Xin Ma,Chenhao Zhao,Jinqi Zhong,Jijie Li,Qi Chen,Mingxiao Han,Hong Du,Qifei Cong,Haifang Zhang

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C3 抗体 [11H9]

Anti-C3 antibody [11H9]

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性