Anti-C3 / C3b抗体[755] (ab11871)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)](https://www.abcam.cn/ps/products/11/ab11871/Images/ab11871-415218-anti-c3-c3b-antibody-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)")
Key features and details
- Mouse monoclonal [755] to C3 / C3b
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human, Cynomolgus monkey
- Isotype: IgG2b
概述
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产品名称
Anti-C3 / C3b抗体[755]
参阅全部 C3 / C3b 一抗 -
描述
小鼠单克隆抗体[755] to C3 / C3b -
宿主
Mouse -
特异性
This antibody reacts with C3 and C3b. See References multiple isoforms detected by WB. -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human, Cynomolgus monkey -
免疫原
Full length native protein (purified) corresponding to Human C3/ C3b.
Database link: P01024 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
755 -
同种型
IgG2b -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11871于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration.
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 25254972
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| IHC-P | (4) |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 25254972 |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
组织特异性
Plasma. -
疾病相关
Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
序列相似性
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
翻译后修饰
C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
- Unigene: 529053 Human
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别名
- Acylation-stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)
Immunohistochemistry analysis of Paraffin embedded section of human tonsil tissue sections labeling C3 / C3b with ab11871 at 1/200.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)](https://www.abcam.cn/ps/products/11/ab11871/Images/ab11871-242676-anti-c3-c3b-antibody-755-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)This image is courtesy of an anonymous Abreviewab11871 staining C3 / C3b in human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/100) for 32 minutes at 37°C. An undiluted biotin-conjugated goat anti-mouse IgG polyclonal was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)](https://www.abcam.cn/ps/products/11/ab11871/Images/ab11871-19120-anti-c3-c3b-antibody-755-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 / C3b antibody [755] (ab11871)This image is courtesy of an anonymous AbreviewImmunohistochemical analysis of Cynomolgus Monkey spleen tissue, staining C3 / C3b with ab11871.
Tissue was fixed with formaldehyde and blocked with blocking solution for 1 hour at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (1/50 in diluent) for 1 hour at 37°C. A biotinylated goat anti-rabbit polyclonal IgG was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab11871 被引用在 26 文献中.
- Huang RY et al. Targeting therapeutic agent against C3b/C4b, SB002, on the inflammation-induced bone loss in experimental periodontitis. J Clin Periodontol 50:657-670 (2023). PubMed: 36632003
- Seifert L et al. The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nat Commun 14:473 (2023). PubMed: 36709213
- Yu B et al. Successful targeting in situ of an oncogenic nuclear antigen by hapten induced tumor associated autoantibodies (iTAA). Sci Rep 13:9902 (2023). PubMed: 37336938
- Rumsey JW et al. Classical Complement Pathway Inhibition in a "Human-On-A-Chip" Model of Autoimmune Demyelinating Neuropathies. Adv Ther (Weinh) 5:N/A (2022). PubMed: 36211621
- Riba M et al. Wasteosomes (corpora amylacea) of human brain can be phagocytosed and digested by macrophages. Cell Biosci 12:177 (2022). PubMed: 36307854