重组Anti-C2抗体[EPR17979] (ab209900)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17979] to C2
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
-
产品名称
Anti-C2抗体[EPR17979]
参阅全部 C2 一抗 -
描述
兔单克隆抗体[EPR17979] to C2 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
阳性对照
- WB: Human serum, plasma and fetal kidney lysate; Mouse serum.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR17979 -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab209900于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
|
说明 |
---|
WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
靶标
-
功能
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
疾病相关
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
序列相似性
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 717 Human
- Entrez Gene: 12263 Mouse
- Omim: 217000 Human
- SwissProt: P06681 Human
- SwissProt: P21180 Mouse
- Unigene: 408903 Human
- Unigene: 283217 Mouse
-
别名
- ARMD14 antibody
- C2 antibody
- C3/C5 convertase antibody
see all
图片
-
All lanes : Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 3 minutes; Lane 2: 30 seconds.
-
Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
数据表及文件
-
SDS download
-
Datasheet download
Certificate of Compliance
文献 (1)
ab209900 被引用在 1 文献中.
- Pei J et al. Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak. BMC Vet Res 18:34 (2022). PubMed: 35031034