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重组RabMAb

重组Anti-C1orf93抗体[EPR13703] (ab180932)

Submit a review Submit a question References (1)
Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
  • Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
  • Immunoprecipitation - Anti-C1orf93 antibody [EPR13703] (ab180932)
  • Anti-C1orf93 antibody [EPR13703] (ab180932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13703] to C1orf93
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-C1orf93抗体[EPR13703]
    参阅全部 C1orf93 一抗

  • 描述

    兔单克隆抗体[EPR13703] to C1orf93

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IP, WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig, Orangutan

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Fetal kidney, HeLa, MCF7 and THP1 cell lines.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR13703

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab180932于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
1/60.
WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
说明
IP
1/60.
WB
1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

靶标

图片

  • Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
    Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
    All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/5000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 21 kDa

  • Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
    Western blot - Anti-C1orf93 antibody [EPR13703] (ab180932)
    Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/1000 dilution + THP1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

  • Immunoprecipitation - Anti-C1orf93 antibody [EPR13703] (ab180932)
    Immunoprecipitation - Anti-C1orf93 antibody [EPR13703] (ab180932)

    Immunoprecipitation of fetal kidney cell line labeling C1orf93 using ab180932 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration: 5% NFDM/TBST



    All lanes : Anti-C1orf93 antibody [EPR13703] (ab180932) at 1/60 dilution

    Lane 1 : fetal kidney cell line at 10 µg
    Lane 2 : Negative control

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution
  • Anti-C1orf93 antibody [EPR13703] (ab180932)
    Anti-C1orf93 antibody [EPR13703] (ab180932)

文献 (1)

发表研究结果有使用 ab180932？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab180932 被引用在 1 文献中.

  • Chen P  et al. Alterations of endogenous pain-modulatory system of the cerebral cortex in the neuropathic pain. iScience 26:106668 (2023). PubMed: 37168579

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