Anti-C1orf77/FOP抗体(ab123603)
Key features and details
- Rabbit polyclonal to C1orf77/FOP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C1orf77/FOP抗体
参阅全部 C1orf77/FOP 一抗 -
描述
兔多克隆抗体to C1orf77/FOP -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human C1orf77/FOP aa 1-100.
Database link: Q9Y3Y2 -
阳性对照
- Whole cell lysate from Jurkat, 293T and HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab123603于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/2500. Predicted molecular weight: 26 kDa.
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| 说明 |
|---|
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WB
1/500 - 1/2500. Predicted molecular weight: 26 kDa. |
靶标
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功能
Plays an important role in the ligand-dependent activation of estrogen receptor target genes (PubMed:19858291). May play a role in the silencing of fetal globin genes (PubMed:20688955). Recruits the 5FMC complex to ZNF148, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (By similarity). Plays an important role in the tumorigenicity of glioblastoma cells. Binds to 5-hydroxymethylcytosine (5hmC) and associates with the methylosome complex containing PRMT1, PRMT5, MEP50 and ERH. The CHTOP-methylosome complex associated with 5hmC is recruited to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).
Required for effective mRNA nuclear export and is a component of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Stimulates DDX39B ATPase and helicase activities. In cooperation with ALYREF/THOC4 enhances NXF1 RNA binding activity (PubMed:23299939). -
组织特异性
Expressed in an erythroid progenitor cell line derived from peripheral blood. Expressed in glioblastoma cells (PubMed:25284789). -
翻译后修饰
Asymmetrically methylated by PRMT1 (PubMed:25284789). Symmetrically methylated by PRMT5. -
细胞定位
Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus speckle. Mostly associated with facultative heterochromatin (By similarity). Localizes to regions surrounding nuclear speckles known as perispeckles in which TREX complex assembly seems to occur (PubMed:23826332). - Information by UniProt
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数据库链接
- Entrez Gene: 426357 Chicken
- Entrez Gene: 738288 Chimpanzee
- Entrez Gene: 616638 Cow
- Entrez Gene: 480140 Dog
- Entrez Gene: 101142690 Gorilla
- Entrez Gene: 26097 Human
- Entrez Gene: 66511 Mouse
- Entrez Gene: 100153064 Pig
see all -
别名
- C1orf77 antibody
- chromatin target of chromatin target of PRMT1 protein antibody
- Chromatin target of PRMT1 antibody
see all
图片
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Western blot - Anti-C1orf77/FOP antibody (ab123603)All lanes : Anti-C1orf77/FOP antibody (ab123603) at 0.4 µg/ml
Lane 1 : Whole cell lysate from Jurkat cells at 50 µg
Lane 2 : Whole cell lysate from Jurkat cells at 15 µg
Lane 3 : Whole cell lysate from 293T cells at 50 µg
Lane 4 : Whole cell lysate from HeLa cells at 50 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab123603 尚未被引用在任何文献中。