Anti-C1orf64抗体(ab121878)
Key features and details
- Rabbit polyclonal to C1orf64
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-C1orf64抗体 -
描述
兔多克隆抗体to C1orf64 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
antigen sequence: HLTRVTPMGG GCLAQARATL PLCRGSVASA SFPVSPLCPQ EVPEAKGKPV KAAPVRSSTW GTVKDSLKAL SSCVCGQ, corresponding to amino acids 91-167 of Human C1orf64. (Q8NEQ6)
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阳性对照
- Human tonsil tissue; C1orf64 over-expression C1orf64 lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab121878于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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| 说明 |
|---|
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
靶标
- Information by UniProt
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数据库链接
- Entrez Gene: 149563 Human
- SwissProt: Q8NEQ6 Human
- Unigene: 29190 Human
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别名
- C1orf64 antibody
- CA064_HUMAN antibody
- ER related factor antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-C1orf64 antibody (ab121878)Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab121878 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1orf64 antibody (ab121878)ab121878 at 1/70 dilution staining C1orf64 in Paraffin-embedded Human Tonsil tissue by Immunohistochemistry. -
Western blot - Anti-C1orf64 antibody (ab121878)All lanes : Anti-C1orf64 antibody (ab121878) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells
Developed using the ECL technique.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab121878 尚未被引用在任何文献中。