AB250554

重组Anti-C16orf70抗体[EPR14035] - BSA and Azide free

Anti-C16orf70 antibody [EPR14035] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal C16orf70 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

查看别名

C16orf6, C16orf70, PHAF1, Phagosome assembly factor 1

2 Images

Supplier Data

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

This data was developed using ab181987, the same antibody clone in a different buffer formulation.

Immunoprecipitation of SH-SY5Y cell lysate, labeling C16orf70 usinf ab181987 at a 1/30 dilution (lane 1) or a negative control (lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/5000 dilution.

All lanes:

Immunoprecipitation - Anti-C16orf70 antibody [EPR14035] (<a href='/products/primary-antibodies/c16orf70-antibody-epr14035-ab181987'>ab181987</a>)

Predicted band size: 48 kDa

false

Supplier Data

Western blot - Anti-C16orf70 antibody [EPR14035] - BSA and Azide free (AB250554)

This data was developed using ab181987, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C16orf70 antibody [EPR14035] (<a href='/products/primary-antibodies/c16orf70-antibody-epr14035-ab181987'>ab181987</a>) at 1/1000 dilution

Lane 1:

Human fetal brain cell lysate at 20 µg

Lane 2:

U87-MG cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

JAR cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 48 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-C16orf70 antibody [EPR14035]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR14035

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

IP, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

ab250554 is the carrier-free version of ab181987.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C16orf70 also known as Chromosome 16 Open Reading Frame 70 codes for a protein with a mass approximately of 17 kDa. This protein is mainly expressed in tissues such as the liver and lungs. C16orf70 may involve various functions in cellular processes but it lacks extensive characterization. Researchers aim to understand its role in different cellular contexts as it may hold significance in tissue-specific functions.

Biological function summary

The actions of C16orf70 suggest involvement in cellular signaling pathways. Studies hint at its participation in protein complexes although exact partners require further identification. Its presence in certain tissues implies a potential role linked to intercellular communication possibly influencing cell growth or immune responses. Further research continues to explore these biological interactions.

Pathways

C16orf70 might engage in critical roles within pathways like the Wnt signaling and cellular senescence. Its interactions with other proteins possibly including those from the Wnt pathway suggest involvement in regulating gene expression and maintaining cellular health. Understanding its precise function in these pathways will provide deeper insights into cell regulation and development.

Researchers link C16orf70 to conditions like cancer and chronic obstructive pulmonary disease (COPD). The protein's altered expression or function may interfere with cellular pathways highlighting associations with oncogenic or inflammatory processes. Studies investigate its connection with proteins involved in these disorders aiming to uncover potential therapeutic targets or biomarkers.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a regulatory role in autophagic activity. In complex with BCAS3, associates with the autophagosome formation site during both non-selective and selective autophagy.

See full target information PHAF1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-C16orf70抗体[EPR14035] - BSA and Azide free