AB250954

重组Anti-C14orf166抗体[EPR15799] - BSA and Azide free

Anti-C14orf166 antibody [EPR15799] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal C14orf166 antibody. Carrier free. Suitable for ICC/IF, IP, WB, IHC-P and reacts with Human, Rat, Mouse samples.

查看别名

C14orf166, CGI-99, RTRAF, CLE7 homolog, CLE, hCLE

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human astrocytoma tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling C14orf166 using ab188326 at 1/100 dilution. A ready to use HRP polymer for rabbit IgG (prediluted) was used as secondary. Counterstain : Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of U87-MG cells labeling C14orf166 using ab188326 at 1/100 dilution. A goat anti rabbit IgG (Alexa Fluor488) at 1/200 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain : DAPI.

Supplier Data

Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of 293T cell lysate labeling C14orf166 using ab188326 at 1/50 dilution (Lane 1). An anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of 293T cell lysate.

All lanes:

Immunoprecipitation - Anti-C14orf166 antibody [EPR15799] (<a href='/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>)

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C14orf166 antibody [EPR15799] (<a href='/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>) at 1/10000 dilution

Lane 1:

Ramos cell lysate at 20 µg

Lane 2:

293T cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

Supplier Data

Western blot - Anti-C14orf166 antibody [EPR15799] - BSA and Azide free (AB250954)

This data was developed using ab188326, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-C14orf166 antibody [EPR15799] (<a href='/products/primary-antibodies/c14orf166-antibody-epr15799-ab188326'>ab188326</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw264.7 cell lysate at 10 µg

Lane 3:

PC12 cell lysate at 10 µg

Lane 4:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

不同偶联物与剂型 (1)

Unconjugated

Anti-C14orf166 antibody [EPR15799]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR15799

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

ICC/IF, IHC-P, WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

ab250954 is the carrier-free version of ab188326.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C14orf166 also known as TIPH2 is a protein encoded by the C14orf166 gene. This protein has a molecular mass of approximately 38 kDa. It plays a role in RNA metabolism particularly in the export of messenger RNA (mRNA) from the nucleus to the cytoplasm. Expression of C14orf166 occurs in various tissues with higher levels often found in actively dividing and metabolically active cells.

Biological function summary

C14orf166 integrates into the transcriptional regulation process by forming part of a complex involved in RNA processing. It interacts with components of the transcription-export (TREX) complex which is essential for coupling mRNA processing with export. This integration allows C14orf166 to assist in maintaining efficient mRNA transport and translation important for normal cellular function and stress response.

Pathways

C14orf166 significantly influences gene expression regulation and participates in the mRNA processing pathways. It interacts with proteins such as Aly/REF and UAP56 within the TREX complex importantly impacting the efficiency of mRNA export. By securing efficient mRNA transport C14orf166 supports critical biological pathways like the unfolded protein response and cellular proliferation.

Aberrant expression or function of C14orf166 has connections to cancer particularly breast cancer. Dysregulation in its function can lead to improper mRNA export contributing to tumorigenesis. Furthermore C14orf166 shows relations to viral infections; for example it intersects with pathways involving Nucleophosmin (NPM1) which are exploited by viruses such as influenza. This makes C14orf166 a potential target for therapeutic strategies aiming to manage these conditions.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

RNA-binding protein involved in modulation of mRNA transcription by Polymerase II (PubMed : 16950395). Component of the tRNA-splicing ligase complex and is required for tRNA ligation (PubMed : 24870230). May be required for RNA transport (PubMed : 24608264).. (Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication (PubMed : 21900157).

See full target information RTRAF

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Abcam Product Promise

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详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-C14orf166抗体[EPR15799] - BSA and Azide free