Anti-C14orf133抗体(ab125084)
Key features and details
- Rabbit polyclonal to C14orf133
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-C14orf133抗体
参阅全部 C14orf133 一抗 -
描述
兔多克隆抗体to C14orf133 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide, corresponding to a region between amino acids 443-493 of Human C14orf133 (NP_071350.2).
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阳性对照
- HeLa, 293T, Jurkat and NIH3T3 whole cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab125084 was affinity purified using an epitope specific to C14orf133 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125084于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
|
WB
1/2000 - 1/10000. Predicted molecular weight: 57 kDa. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Plays a role in lysosomal trafficking. May play a role in epithelial polarization through stabilization of apical membrane protein content, possibly via the RAB11A-dependent apical recycling pathway. Also involved in direct or indirect transcriptional regulation of E-cadherin. -
疾病相关
Defects in VIPAR are the cause of arthrogryposis-renal dysfunction-cholestasis syndrome type 2 (ARCS2) [MIM:613404]. ARCS2 is an autosomal recessive multisystem disorder, characterized by neurogenic arthrogryposis multiplex congenita, renal tubular dysfunction and neonatal cholestasis with bile duct hypoplasia and low gamma glutamyl transpeptidase activity. Platelet dysfunction is common. Note=In liver, CEACAM5 and ABCB11 are mislocalized and E-cadherin expression is decreased. -
序列相似性
Belongs to the VIPAR family. -
细胞定位
Early endosome. Recycling endosome. Late endosome. - Information by UniProt
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数据库链接
- Entrez Gene: 63894 Human
- Entrez Gene: 104799 Mouse
- Omim: 613401 Human
- SwissProt: Q9H9C1 Human
- SwissProt: Q8BGQ1 Mouse
- Unigene: 16157 Human
- Unigene: 208750 Mouse
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别名
- Apical basolateral polarity regulator antibody
- FLJ12707 antibody
- hSPE-39 antibody
see all
图片
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Western blot - Anti-C14orf133 antibody (ab125084)All lanes : Anti-C14orf133 antibody (ab125084) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-C14orf133 antibody (ab125084)Detection of C14orf133 by Western Blot of Immunprecipitate.
ab125084 at 1µg/ml staining C14orf133 in HeLa whole cell lysate immunoprecipitated using ab125084 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab125084 被引用在 2 文献中.
- Liu RJY et al. The Sec1-Munc18 protein VPS33B forms a uniquely bidirectional complex with VPS16B. J Biol Chem 299:104718 (2023). PubMed: 37062417
- Dai J et al. Vps33b regulates Vwf-positive vesicular trafficking in megakaryocytes. J Pathol 240:108-19 (2016). PubMed: 27319744