AB167413

重组Anti-C11B2/CYP11B2抗体[EPR10495]

Anti-C11B2/CYP11B2 antibody [EPR10495]

20ul selling size
RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal C11B2/CYP11B2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 6 publications.

查看别名

Aldosterone synthase, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase, ALDOS, CYP11B2

4 Images

Unknown

Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (AB167413)

Detection of C11B2/CYP11B2 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab167413 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413)

Predicted band size: 58 kDa

false

Lab

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (AB167413)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

Lane 1:

Human adrenal gland tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Lane 5:

Human liver tissue lysate at 20 µg

Lane 6:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 7:

HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 58 kDa

Observed band size: 48 kDa

false

Exposure time: 20s

Unknown

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (AB167413)

All lanes:

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

Lane 1:

Human adrenal gland tissue lysate at 10 µg

Lane 2:

NIH:OVCAR-3 cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 58 kDa

false

Lab

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (AB167413)

All lanes:

Western blot - Anti-C11B2/CYP11B2 antibody [EPR10495] (ab167413) at 1/1000 dilution

Lane 1:

MCF-7 cell lysate with Human C11B2/CYP11B2 peptide

Lane 2:

MCF-7 cell lysate with Human C11B1/CYP11B2 peptide

Lane 3:

MCF-7 cell lysate with no blocking peptide

Predicted band size: 58 kDa

Observed band size: 48 kDa

false

Exposure time: 3s

不同偶联物与剂型 (1)

Carrier free

Anti-C11B2/CYP11B2 antibody [EPR10495] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR10495

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen used for this product shares 5 consecutive identical amino acids with family members like CYP11B1, CYP3A4, CYP3A43, CYP3A5, CYP3A7. Cross-reactivity with these proteins have not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C11B2 also known as CYP11B2 is a highly specific enzyme that plays an essential role in the biosynthesis of aldosterone. Also referred to as aldosterone synthase this cytochrome P450 family member is encoded by the CYP11B2 gene. It has a molecular mass of approximately 58 kDa. Its expression occurs mainly in the adrenal cortex particularly within the zona glomerulosa cells where it catalyzes the final steps in the production of aldosterone from cholesterol.

Biological function summary

CYP11B2 converts 11-deoxycorticosterone to corticosterone which is then converted to aldosterone. This enzyme functions independently and is not part of any larger protein complexes. It undertakes its role in the mitochondria of adrenal gland cells where it serves as an important factor in the corticosteroid synthesis pathway. Its activity is regulated via several factors including angiotensin II and potassium ion concentration to maintain homeostatic balance within the body.

Pathways

CYP11B2 is an integral part of the steroidogenic pathway specifically within the branch that leads to mineralocorticoid biosynthesis. This pathway involves the conversion of cholesterol to steroid hormones and is closely linked to the renin-angiotensin-aldosterone system (RAAS) which governs blood pressure and fluid balance. Besides its relationship with cholesterol side-chain cleavage enzyme (CYP11A1) CYP11B2 interacts with other proteins like 3-beta-hydroxysteroid dehydrogenase in facilitating steroidogenesis.

Abnormalities in CYP11B2 expression or function can lead to aldosterone-related conditions such as primary aldosteronism and Addison’s disease. Primary aldosteronism results from overproduction of aldosterone causing hypertension and electrolyte imbalances while its deficiency contributes to Addison's disease. These disorders can also involve connections with proteins like CYP17A1 which is another key player in steroid biosynthesis and whose disruptions can influence CYP11B2's activity or expression amplifying disease pathology.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure (PubMed : 11856349, PubMed : 12530636, PubMed : 1518866, PubMed : 15356073, PubMed : 1594605, PubMed : 1775135, PubMed : 22446688, PubMed : 23322723, PubMed : 9814482, PubMed : 9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone (PubMed : 11856349, PubMed : 12530636, PubMed : 1518866, PubMed : 1594605, PubMed : 1775135, PubMed : 22446688, PubMed : 23322723, PubMed : 9814506). Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension (PubMed : 15356073, PubMed : 9814482). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed : 11856349, PubMed : 1594605, PubMed : 23322723, PubMed : 9814506). Could also be involved in the androgen metabolic pathway (Probable).

See full target information Cytochrome P450 11B2, mitochondrial

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0314943 PubMed39946402

2025

Meriones unguiculatus serves as a spontaneous primary aldosteronism rodent model.

Applications

Unspecified application

Species

Unspecified reactive species

Mei You,Zongshi Lu,Bowen Wang,Min Liu,Qing Zhou,Li Li,Dan Tong,Yu Zhao,Hexuan Zhang,Zhongping Bai,Lijuan Wang,Tingbing Cao,Peng Gao,Zhencheng Yan,Zhiming Zhu

Acta physiologica (Oxford, England) 237:e13899 PubMed36264268

2022

Collecting duct renin regulates potassium homeostasis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanming Xu,Yanting Chen,Nirupama Ramkumar,Chang-Jiang Zou,Curt D Sigmund,Tianxin Yang

Cells 11: PubMed36230992

2022

Di-n-Butyl Phthalate and Its Monoester Metabolite Impairs Steroid Hormone Biosynthesis in Human Cells: Mechanistic In Vitro Studies.

Applications

Unspecified application

Species

Unspecified reactive species

Liselott Källsten,Paula Pierozan,Jonathan W Martin,Oskar Karlsson

Frontiers in endocrinology 11:561370 PubMed33133015

2020

Adropin Stimulates Proliferation and Inhibits Adrenocortical Steroidogenesis in the Human Adrenal Carcinoma (HAC15) Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Ewelina Stelcer,Paulina Milecka,Hanna Komarowska,Karol Jopek,Marianna Tyczewska,Marta Szyszka,Marta Lesniczak,Wiktoria Suchorska,Karlygash Bekova,Beata Szczepaniak,Marek Ruchala,Marek Karczewski,Tomasz Wierzbicki,Witold Szaflarski,Ludwik K Malendowicz,Marcin Rucinski

International journal of experimental pathology 99:77-86 PubMed29665181

2018

MiR-193a-3p functions as a tumour suppressor in human aldosterone-producing adrenocortical adenoma by down-regulating CYP11B2.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxi Zhang,Xiaofeng Zou,Quanliang Liu,Tianpeng Xie,Ruohui Huang,Huan Kang,Changfu Lai,Jiaxing Zhu

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 48:1087-94 PubMed26421867

2015

POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells.

Applications

Species

Unspecified reactive species

M M França,N P Abreu,T A M Vrechi,C F Lotfi

View all publications

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重组Anti-C11B2/CYP11B2抗体[EPR10495]