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重组RabMAb

重组Anti-C10抗体[EPR23475-105] - BSA and Azide free (ab275039)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
  • Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23475-105] to C10 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, IHC-Fr, IP
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-C10抗体[EPR23475-105] - BSA and Azide free
    参阅全部 C10 一抗

  • 描述

    兔单克隆抗体[EPR23475-105] to C10 - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), IHC-P, IHC-Fr, IPmore details
    不适用于: ICC/IF or WB

  • 种属反应性

    与反应: Mouse, Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse spleen and lung tissue. IHC-Fr: Mouse small intestine and colon tissue. Flow Cyt (intra): P388D1 cells (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h). IP: Mouse small intestine tissue lysate.

  • 常规说明

    ab275039 is the carrier-free version of ab275025.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR23475-105

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab275039于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
Use at an assay dependent concentration.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IP
Use at an assay dependent concentration.
应用说明
Is unsuitable for ICC/IF or WB.

靶标

  • 相关性

    CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • C10 antibody
      • CCL 6 antibody
      • chemokine (C-C motif) ligand 6 antibody
      • Chemokine C C motif Ligand 6 antibody
      • MRP1 antibody
      • SCYA6 antibody
      • Small Inducible Cytokine A6 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse spleen (PMID: 15770051). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse small intestine tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse small intestine (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    • Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Flow Cytometry (Intracellular) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation. 

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized P388D1 (Mouse lymphoma monocyte; macrophage) (treated with 1ug/ml lipopolysaccharide (LPS) for 4h then add 300ng/ml Brefeldin A for 16h) (Red) / Untreated control (Green) cells labelling C10 with ab275025 at 1/500 dilution (0.1ug) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). 

      A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

       

       

    • Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Western blot - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      All lanes : Anti-C10 antibody [EPR23475-105] (ab275025) at 1/1000 dilution

      Lane 1 : His-tagged mouse C10 recombinant protein, 20ng
      Lane 2 : His-tagged mouse MIP-1 gamma (CCL9) recombinant protein (aa 22-122), 50ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 13 kDa



      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      This product does not recognize CCL9.

    • Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Immunoprecipitation - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      C10 was immunoprecipitated from 0.35 mg Mouse small intestine tissue lysate with ab275025 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab275025 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/1000 dilution.

      Lane 1: Mouse small intestine tissue lysate 10 ug

      Lane 2: ab275025 IP in Mouse small intestine tissue lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab275025 in mouse small intestine tissue lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      The expression profile observed is consistent with what has been described in the literature (PMID: 19812544).

    • Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Immunohistochemistry (Frozen sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon tissue labeling C10 with ab275025 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse colon (PMID: 7579352). The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 (2 ug/ml) dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

      This data was developed using ab275025, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling C10 with ab275025 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on mouse lung (PMID: 14734772). The section was incubated with ab275025 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)
      Anti-C10 antibody [EPR23475-105] - BSA and Azide free (ab275039)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab275039？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab275039 尚未被引用在任何文献中。

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