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使用敲除细胞株进行验证重组RabMAb

重组Anti-c-Rel抗体[EPR2559(2)] (ab133251)

Reviews (3) Submit a question References (4)
Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
  • Anti-c-Rel antibody [EPR2559(2)] (ab133251)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2559(2)] to c-Rel
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-c-Rel抗体[EPR2559(2)]
    参阅全部 c-Rel 一抗

  • 描述

    兔单克隆抗体[EPR2559(2)] to c-Rel

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: HAP1, HuT-78, and HeLa cell lysates. IP: HeLa cell lysate.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab133251于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/10000 - 1/50000.
IP
1/10 - 1/100.
说明
WB
1/1000. Predicted molecular weight: 68 kDa.

For unpurified use at 1/10000 - 1/50000.
IP
1/10 - 1/100.

靶标

  • 功能

    Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.

  • 序列相似性

    Contains 1 RHD (Rel-like) domain.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Avian reticuloendotheliosis antibody
      • C REL antibody
      • C Rel protein antibody
      • c Rel proto oncogene protein antibody
      • Oncogene REL antibody
      • Oncogene REL avian reticuloendotheliosis antibody
      • Proto-oncogene c-Rel antibody
      • REL antibody
      • REL_HUMAN antibody
      • v rel avian reticuloendotheliosis viral oncogene homolog antibody
      • v rel reticuloendotheliosis viral oncogene homolog antibody
      • V rel reticuloendotheliosis viral oncogene homolog (avian) antibody
      see all

    图片

    • Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Immunoprecipitation - Anti-c-Rel antibody [EPR2559(2)] (ab133251)

      Purified ab133251 at 1/20 dilution (0.6µg) immunoprecipitating c-Rel in HeLa whole cell lysate.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab133251 + HeLa whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133251 in HeLa whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 78 kDa
      Fresh lysate is required to avoid protein degradation.

    • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      All lanes : Anti-c-Rel antibody [EPR2559(2)] (ab133251) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 68 kDa

    • Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Western blot - Anti-c-Rel antibody [EPR2559(2)] (ab133251)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: c-Rel knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab133251 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab133251 was shown to recognize c-Rel when c-Rel knockout samples were used, along with additional cross-reactive bands. Wild-type and c-Rel knockout samples were subjected to SDS-PAGE. ab133251 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Anti-c-Rel antibody [EPR2559(2)] (ab133251)
      Anti-c-Rel antibody [EPR2559(2)] (ab133251)

    文献 (4)

    发表研究结果有使用 ab133251？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab133251 被引用在 4 文献中.

    • Liu HF  et al. Dipeptidyl peptidase-8 induces sorafenib resistance via binding with c-Rel to mediate NF-κB signaling in hepatocellular carcinoma. Cell Biol Int 46:213-221 (2022). PubMed: 34719075
    • Zhu H  et al. Knockdown of RSAD2 attenuates B cell hyperactivity in patients with primary Sjögren's syndrome (pSS) via suppressing NF-?b signaling pathway. Mol Cell Biochem 476:2029-2037 (2021). PubMed: 33512636
    • Zhou Z  et al. LncRNA XIST promotes inflammation by downregulating GRa expression in the adenoids of children with OSAHS. Exp Ther Med 21:500 (2021). PubMed: 33791009
    • Zhang C  et al. Activation of TNF-a/NF-?B axis enhances CRL4BDCAF11E3 ligase activity and regulates cell cycle progression in human osteosarcoma cells. Mol Oncol N/A:N/A (2018). WB . PubMed: 29377600

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    Western blot abreview for Anti-c-Rel antibody [EPR2559(2)]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (mouse neural stem cell)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    50 µg
    Specification
    mouse neural stem cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 15°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Robert Kupp

    Verified customer

    提交于 Sep 17 2019

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