AB32412

Anti-C Reactive Protein 抗体 [Y284]

Name: C Reactive Protein抗体[Y284] (ab32412)| Abcam中文官网
Brand: Abcam Limited
SKU: AB32412
Price: 1442 CNY
Availability: InStock
Rating: 5 (6 reviews)

Anti-C Reactive Protein antibody [Y284]

RabMAb
Recombinant
20ul selling size
了解详情

(6 Reviews)

(43 Publications)

Anti-C Reactive Protein antibody [Y284] (ab32412) is a rabbit monoclonal antibody detecting C Reactive Protein in Western Blot, IP, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
- Trusted since 2006

查看别名

PTX1, CRP, C-reactive protein

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded) analysis of human normal liver tissue labelling C Reactive Protein using unpurified ab32412 at a dilution of 1/100.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling C Reactive Protein with purified ab32412 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051 a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C Reactive Protein antibody [Y284] (AB32412)

Immunohistochemical analysis of formalin fixed paraffin embedded human liver labelling C Reactive Protein with ab32412 at a concentration of 0.01µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32 mins. ab32412 anti-C Reactive Protein antibody [Y284] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

Unknown

Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (AB32412)

ab32412 (purified) at 1/30 immunoprecipitating C Reactive Protein in HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate.

Lane 1 (input) : HepG2 whole cell lysate (10μg)

Lane 2 (+) : ab32412 + HepG2 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab32412 in HepG2 whole cell lysate.

For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-C Reactive Protein antibody [Y284] (ab32412)

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

Lab

Western blot - Anti-C Reactive Protein antibody [Y284] (AB32412)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/2000 dilution

Lane 1:

Human serum at 20 µg

Lane 2:

Human plasma at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/50000 dilution

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

Unknown

Western blot - Anti-C Reactive Protein antibody [Y284] (AB32412)

All lanes:

Western blot - Anti-C Reactive Protein antibody [Y284] (ab32412) at 1/1000 dilution

All lanes:

Human serum

Predicted band size: 25 kDa

Observed band size: 27 kDa

false

不同偶联物与剂型 (9)

Carrier free

Anti-C Reactive Protein antibody [Y284] - BSA and Azide free
HRP

HRP Anti-C Reactive Protein antibody [Y284]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-C Reactive Protein antibody [Y284]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-C Reactive Protein antibody [Y284]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-C Reactive Protein antibody [Y284]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-C Reactive Protein antibody [Y284]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-C Reactive Protein antibody [Y284]
Carrier free

Anti-C Reactive Protein antibody [Y284] - Low endotoxin, Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

Y284

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IP, IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

表位

ab32412 reacts with an epitope located in the C terminal region of C Reactive Protein.

特异性

This antibody recognises C Reactive Protein. This antibody is predicted to detect the splice isoform 2 as well as isoform 1.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

What is this antibody validated in?
Anti-C Reactive Protein antibody [Y284] (ab32412) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of C Reactive Protein?
Anti-C Reactive Protein [Y284] (ab32412) specifically detects a band for C Reactive Protein (UniProt: P02741) at a molecular weight of 25kDa.

Trusted by the scientific community
Anti-C Reactive Protein [Y284] (ab32412) was first used in a scientific publication in 2006 and has been cited over 30 times in peer-reviewed journals.

Reviewed by scientists
Anti-C Reactive Protein [Y284] (ab32412) has over 5 independent reviews from customers.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [Y284] also available for your convenience: ab32412, Carrier free - ab157242, Carrier free - ab271830, HRP - ab307473, Alexa Fluor® 647 - ab307519, Alexa Fluor® 594 - ab311801, Alexa Fluor® 568 - ab313084, Alexa Fluor® 555 - ab313278, Alexa Fluor® 488 - ab314471, Alexa Fluor® 750 - ab321600

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.

Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

See full target information CRP

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文献 (43)

Recent publications for all applications. Explore the full list and refine your search

European stroke journal 10:892-901 PubMed39910895

2025

C-reactive protein expression in acute ischemic stroke blood clots: Implications for etiology.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Liu,Cansu Sahin,Nazan Güner Sak,Alice Giraud,Pierluca Messina,Franz Bozsak,Jean Darcourt,Federico Sacchetti,Anne-Christine Januel,Guillaume Bellanger,Jorge Pagola,Jesus Juega,Hirotoshi Imamura,Tsuyoshi Ohta,Laurent Spelle,Vanessa Chalumeau,Uros Mircic,Predrag Stanarčević,Ivan Vukašinović,Marc Ribo,Nobuyuki Sakai,Christophe Cognard,Karen Doyle

Frontiers in immunology 15:1475051 PubMed39737187

2024

CRP deposition in human abdominal aortic aneurysm is associated with transcriptome alterations toward aneurysmal pathogenesis: insights from spatial whole transcriptomic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Hee Young Seok,Joon Seo Lim,Jiwon Koh,Jeong Mo Bae,Chong Jai Kim,Ga-Hyeon Ryu,You Jung Ok,Jae-Sung Choi,Chung-Hyun Cho,Se Jin Oh

Diagnostics (Basel, Switzerland) 14: PubMed39061609

2024

Androgen-Induced, β-Catenin-Activated Hepatocellular Adenomatosis with Spontaneous External Rupture.

Applications

Unspecified application

Species

Unspecified reactive species

Jialing Huang,Towhid Ali,David M Feldman,Neil D Theise

International endodontic journal 57:406-415 PubMed38243897

2024

Reduced C-reactive protein levels after root canal treatment in clinically healthy young apical periodontitis individuals at cardiovascular risk. A prospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Mauricio Garrido,María José Bordagaray,Catalina Schweitzer,Joaquín Lucero-Mora,Montserrat Reyes,Elizabeth Pellegrini,Patricia Hernández-Ríos,Alejandra Fernández,Marcela Hernández

Asia-Pacific journal of clinical oncology 21:77-86 PubMed37415393

2023

C-reactive protein is a prognostic biomarker in pancreatic ductal adenocarcinoma patients.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa F Bonazzi,Lauren G Aoude,Sandra Brosda,Julia J Bradford,James M Lonie,Kelly A Loffler,Michael G Gartside,Kalpana Patel,Pamela Mukhopadhyay,Colm Keane,Val Gebski,James G Kench,David Goldstein,Nicola Waddell,Andrew P Barbour

iScience 26:106247 PubMed36926653

2023

Deciduous tooth biomarkers reveal atypical fetal inflammatory regulation in autism spectrum disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Dani Dumitriu,Elena Baldwin,Roozie J J Coenen,Luke A Hammond,Darcy S Peterka,Lynne Heilbrun,Richard E Frye,Raymond Palmer,Hjalmar Nobel Norrman,Anna Fridell,Karl Lundin Remnelius,Johan Isaksson,Christine Austin,Paul Curtin,Sven Bölte,Manish Arora

EBioMedicine 77:103903 PubMed35220044

2022

Alzheimer's disease protease-containing plasma extracellular vesicles transfer to the hippocampus via the choroid plexus.

Applications

Unspecified application

Species

Unspecified reactive species

Jung-Hyun Lee,Christian Ostalecki,Timo Oberstein,Stefan Schierer,Elisabeth Zinser,Martin Eberhardt,Katja Blume,Bianca Plosnita,Lena Stich,Heiko Bruns,Roland Coras,Julio Vera-Gonzales,Manuel Maler,Andreas S Baur

Communications medicine 2:11 PubMed35603298

2022

Genomic analysis of focal nodular hyperplasia with associated hepatocellular carcinoma unveils its malignant potential: a case report.

Applications

Unspecified application

Species

Unspecified reactive species

Caner Ercan,Mairene Coto-Llerena,John Gallon,Lana Fourie,Mattia Marinucci,Gabriel F Hess,Jürg Vosbeck,Stephanie Taha-Mehlitz,Tuyana Boldanova,Marie-Anne Meier,Alexandar Tzankov,Matthias S Matter,Martin H K Hoffmann,Luca Di Tommaso,Markus von Flüe,Charlotte K Y Ng,Markus H Heim,Savas D Soysal,Luigi M Terracciano,Otto Kollmar,Salvatore Piscuoglio

PloS one 16:e0245361 PubMed34428207

2021

CRP immunodeposition and proteomic analysis in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Na Kim,Jiyoung Yu,Joon Seo Lim,Hwangkyo Jeong,Chong Jai Kim,Jae-Sung Choi,So Ra Kim,Hee-Sung Ahn,Kyunggon Kim,Se Jin Oh

Archives of pathology & laboratory medicine 146:220-226 PubMed34086854

2021

HNF-1β is a More Sensitive and Specific Marker Than C-Reactive Protein for Identifying Biliary Differentiation in Primary Hepatic Carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Pallavi A Patil,Tamar Taddei,Dhanpat Jain,Xuchen Zhang

View all publications

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Anti-C Reactive Protein 抗体 [Y284]