Anti-c-Myc (phospho T58)抗体(ab28842)
Key features and details
- Rabbit polyclonal to c-Myc (phospho T58)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Myc (phospho T58)抗体
参阅全部 c-Myc 一抗 -
描述
兔多克隆抗体to c-Myc (phospho T58) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide within Human c-Myc aa 1-100 (phospho T58). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P01106 -
阳性对照
- Ovarian cancer cell lysate, breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28842于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 49 kDa.
|
| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use a concentration of 1 µg/ml. |
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WB
1/500 - 1/1000. Predicted molecular weight: 49 kDa. |
靶标
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功能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
疾病相关
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
细胞定位
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 4609 Human
- Entrez Gene: 17869 Mouse
- Omim: 190080 Human
- SwissProt: P01106 Human
- SwissProt: P01108 Mouse
- Unigene: 202453 Human
- Unigene: 2444 Mouse
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形式
c-Myc is also expressed in the cytoplasm. -
别名
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
图片
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Western blot - Anti-c-Myc (phospho T58) antibody (ab28842)Lane 1 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution
Lane 2 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized non-
phosphopeptide
)
Lane 3 : Anti-c-Myc (phospho T58) antibody (ab28842) at 1/500 dilution ( preincubated with synthesized
phosphopeptide
)
Lanes 1 & 3 : human ovarian cancer cell lysate
Lane 2 : human ovarian cancer cell lysate
Predicted band size: 49 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Additional bands at: 120 kDa, 55 kDa, 86 kDa. We are unsure as to the identity of these extra bands. -
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho T58) antibody (ab28842)ICC/IF image of ab28864 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28864, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho T58) antibody (ab28842)Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using Myc (Phospho-Thr58) Antibody; Right: The same antibody preincubated with synthesized phosphopeptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (29)
ab28842 被引用在 29 文献中.
- Xiong Y et al. Small molecule Z363 co-regulates TAF10 and MYC via the E3 ligase TRIP12 to suppress tumour growth. Clin Transl Med 13:e1153 (2023). PubMed: 36639831
- Macaya I et al. Signature-driven repurposing of Midostaurin for combination with MEK1/2 and KRASG12C inhibitors in lung cancer. Nat Commun 14:6332 (2023). PubMed: 37816716
- Stengel S et al. Suppression of MYC by PI3K/AKT/mTOR pathway inhibition in combination with all-trans retinoic acid treatment for therapeutic gain in acute myeloid leukaemia. Br J Haematol 198:338-348 (2022). PubMed: 35468223
- Waters AM et al. Targeting p130Cas- and microtubule-dependent MYC regulation sensitizes pancreatic cancer to ERK MAPK inhibition. Cell Rep 35:109291 (2021). PubMed: 34192548
- Choi YW et al. Senescent Tumor Cells Build a Cytokine Shield in Colorectal Cancer. Adv Sci (Weinh) 8:2002497 (2021). PubMed: 33643790