Anti-c-Myc (phospho S62) 抗体 [EPR17924]
Anti-c-Myc (phospho S62) antibody [EPR17924]
- RabMAb
- Recombinant
- Lab Essentials
- 20ul selling size
- 了解详情
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(58 Publications)
Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) is a rabbit monoclonal antibody detecting c-Myc in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, Dot Blot. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 40 publications
查看别名
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
- Flow Cyt (Intra)
Lab
Flow Cytometry (Intracellular) - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with purified ab185656 at 1/150 dilution (red). The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Immunohistochemical analysis of paraffin-embedded Human endometrium cancer tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on cancer cells of Human endometrial cancer is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling c-Myc (phospho S62) with ab185656 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).
Confocal image showing nuclear staining on HeLa cells.
The staining decreased after blocking with phospho peptide (100μg/ml) overnight.
The control peptide is a non-phospho peptide.
The nuclear counter stain is DAPI (blue).
Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows :
-ve control 1 : ab185656 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear and cytoplasmic staining on mouse spleen is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling c-Myc (phospho S62) with ab185656 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution.
Nuclear staining on rat testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- WB
Supplier Data
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) at 1/2000 dilution
Lane 1:
NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg
Lane 2:
C6 (Rat glial tumor cells) whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 57 kDa
false
Exposure time: 3min
- IP
Supplier Data
Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
c-Myc (phospho S62) was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 200nM TPA for 10 minutes with ab185656 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab185656 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution.
Lane 1 : HeLa whole cell lysate treated with 200nM TPA for 10 minutes,10 μg (Input).
Lane 2 : ab185656 IP in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab185656 in HeLa whole cell lysate treated with 200nM TPA for 10 minutes.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
All lanes:
Immunoprecipitation - Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656)
Predicted band size: 48 kDa
Observed band size: 58 kDa
false
Exposure time: 5s
- WB
Supplier Data
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) at 1/5000 dilution
Lane 1:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2:
HeLa (Human epithelial cells from cervix adenocarcinoma) treated with Lambda Phosphatase whole cell lysate at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 57 kDa
false
Exposure time: 1min
- WB
Lab
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Blocking/Dilution buffer : 5% NFDM/TBST.
All lanes:
Western blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) at 1/5000 dilution
Lane 1:
Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg
Lane 2:
HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes. at 10 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 48 kDa
Observed band size: 57 kDa
false
Exposure time: 30s
- Dot
Supplier Data
Dot Blot - Anti-c-Myc (phospho S62) antibody [EPR17924] (AB185656)
Dot blot analysis of c -Myc (phospho T58) peptide (Lane 1), c-Myc non-phospho peptide (a control peptide for c-Myc phospho T58) (Lane 2), c-Myc (phospho S62) peptide (Lane 3), and c-Myc non-phospho peptide (a control peptide for c-Myc phospho S62) (Lane 4), labeled using ab185656 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer : 5% NFDM/TBST.
Lanes 1, 2 and 4 are control peptides, lane 3 contains the immunogen peptide.
Exposure time=3 minutes.
反应性数据
产品详情
What is this antibody validated in?
Anti-c-Myc (phospho S62) antibody [EPR17924] (ab185656) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Dot Blot in Human, Mouse, Rat samples.
What is the molecular weight of c-Myc?
Anti-c-Myc (phospho S62) [EPR17924] (ab185656) specifically detects a band for c-Myc (UniProt: P01106) at a molecular weight of 48kDa.
Trusted by the scientific community
Anti-c-Myc (phospho S62) [EPR17924] (ab185656) was first used in a scientific publication in 2015 and has been cited over 40 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR17924] also available for your convenience: ab185656, Carrier free - ab232691, PE - ab305731, APC - ab305732, HRP - ab305733, Alkaline Phosphatase - ab308652, Alexa Fluor® 488 - ab309626, Alexa Fluor® 647 - ab309992, Alexa Fluor® 594 - ab310355, Alexa Fluor® 555 - ab311877, Alexa Fluor® 568 - ab312346, Alexa Fluor® 750 - ab321295
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
性能和储存信息
形式
纯化工艺
存储溶液
运输条件
推荐的短期储存时间
推荐的短期储存条件
推荐的长期储存条件
分装信息
储存信息
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
Pathways
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
产品实验方案
- Visit the General protocols
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靶点信息
文献 (58)
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