重组Anti-c-Myb抗体[EPR718(2)] (ab109127)
![Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.cn/ps/products/109/ab109127/Images/ab109127-296659-antic-myb-antibody-epr7182-western-blot-human-hap1.jpg "Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR718(2)] to c-Myb
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-c-Myb抗体[EPR718(2)]
参阅全部 c-Myb 一抗 -
描述
兔单克隆抗体[EPR718(2)] to c-Myb -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat and MOLT4 cell lysates; Human colon tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR718(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109127于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 72 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 72 kDa. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4602 Human
- Omim: 189990 Human
- SwissProt: P10242 Human
- Unigene: 606320 Human
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别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: c-Myb knockout HAP1 whole cell lysate (20 µg)Lanes 1 - 2: Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. Ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.cn/ps/products/109/ab109127/Images/ab109127-121724-anti-c-myb-antibody-epr7182-western-blot.jpg)
Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)All lanes : Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution
Lane 1 : Jurkat cell lysates
Lane 2 : MOLT4 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 72 kDa -
![Anti-c-Myb antibody [EPR718(2)] (ab109127)](https://www.abcam.cn/ps/products/109/ab109127/Images/ab109127-4-benefits-of-recombinant-antibodies.png)
Anti-c-Myb antibody [EPR718(2)] (ab109127)
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab109127 被引用在 10 文献中.
- Srivastava SK et al. MYB interacts with androgen receptor, sustains its ligand-independent activation and promotes castration resistance in prostate cancer. Br J Cancer 126:1205-1214 (2022). PubMed: 34837075
- Wang Y & Wu Z The Clinical Significance and Transcription Regulation of a DNA Damage Repair Gene, SMC4, in Low-Grade Glioma via Integrated Bioinformatic Analysis. Front Oncol 11:761693 (2021). PubMed: 34868977
- Yi T et al. LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission. Cell Death Dis 11:832 (2020). PubMed: 33028809
- Walf-Vorderwülbecke V et al. Targeting acute myeloid leukemia by drug-induced c-MYB degradation. Leukemia 32:882-889 (2018). PubMed: 29089643
- Morrison TA et al. A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression. Blood Cells Mol Dis 69:1-9 (2018). PubMed: 29227829
- Fan J et al. A novel role of CKIP-1 in promoting megakaryocytic differentiation. Oncotarget 8:30138-30150 (2017). PubMed: 28404913
- Li L et al. Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer. Sci Signal 7:ra47 (2014). PubMed: 24847116
- Cyr AR et al. TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis. Oncogene N/A:N/A (2014). PubMed: 24469049
- Baron BW et al. GFI1B, EVI5, MYB-Additional genes that cooperate with the human BCL6 gene to promote the development of lymphomas. Blood Cells Mol Dis N/A:N/A (2013). IHC . PubMed: 23910958
- Ebata A et al. Oestrogen-induced genes in ductal carcinoma in situ: their comparison with invasive ductal carcinoma. Endocr Relat Cancer 19:485-96 (2012). IHC-P ; Human . PubMed: 22569827