AB109127

Anti-c-Myb 抗体 [EPR718(2)]

Anti-c-Myb antibody [EPR718(2)]

RabMAb
Recombinant
KO Validated
了解详情

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(11 Publications)

Rabbit Recombinant Monoclonal c-Myb antibody. Suitable for WB and reacts with Human samples. Cited in 11 publications.

查看别名

Transcriptional activator Myb, Proto-oncogene c-Myb, MYB

2 Images

Lab

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : c-Myb knockout HAP1 whole cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab109127 observed at 75 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab109127 was shown to specifically react with c-Myb in wild-type HAP1 cells as signal was lost in c-Myb knockout cells. Wild-type and c-Myb knockout samples were subjected to SDS-PAGE. ab109127 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127)

Predicted band size: 72 kDa

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Unknown

Western blot - Anti-c-Myb antibody [EPR718(2)] (AB109127)

All lanes:

Western blot - Anti-c-Myb antibody [EPR718(2)] (ab109127) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

MOLT4 cell lysates at 10 µg

Predicted band size: 72 kDa

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不同偶联物与剂型 (1)

Carrier free

Anti-c-Myb antibody [EPR718(2)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR718(2)

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The c-Myb protein also known as Myb MYB or c-myb acts mechanically as a transcription factor that regulates the expression of target genes. It plays a role in cellular processes like proliferation differentiation and apoptosis by binding to specific DNA sequences. c-Myb has a molecular mass of roughly 75 kDa. It predominantly expresses in hematopoietic cells where it significantly influences gene transcription activity. Also it appears in some epithelial tissues indicating its versatile role in different cell types.

Biological function summary

The protein serves as a master regulator in the development of various cell types. It is not part of a traditional complex but interacts with other proteins to exert its function such as binding with co-activators or repressors to modulate gene expression. c-Myb controls the transcription of genes that are critical for cell cycle regulation and differentiation especially in the context of blood cell lineages. The protein is particularly influential in the production of red blood cells and white blood cells.

Pathways

C-Myb fits integrally into known hematopoietic signaling cascades and the Wnt signaling pathway. It works alongside other proteins like cyclin-dependent kinase (CDK) family members and transcription factor GATA-1 to modulate lineage-specific gene expression. Through its regulatory actions c-Myb influences the activation of downstream effectors that propagate signals essential for maintaining cellular functions within these pathways. The modulation of these pathways underlines its role in cell fate decisions.

Studies link c-Myb to several forms of leukemia and colon cancer. In leukemia aberrant expression of c-Myb disrupts normal hematopoiesis contributing to the uncontrolled proliferation of immature blood cells. The abnormal interactions between c-Myb and proteins like Bcl-2 can lead to enhanced cellular survival further promoting malignant conditions. In colon cancer c-Myb overexpression influences tumor growth and progression often acting alongside other oncogenic factors to enhance disease severity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

See full target information MYB

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文献 (11)

Recent publications for all applications. Explore the full list and refine your search

Blood 139:1659-1669 PubMed35007327

2022

Genome-wide association study on 13 167 individuals identifies regulators of blood CD34+cell levels.

Applications

Unspecified application

Species

Unspecified reactive species

Aitzkoa Lopez de Lapuente Portilla,Ludvig Ekdahl,Caterina Cafaro,Zain Ali,Natsumi Miharada,Gudmar Thorleifsson,Kristijonas Žemaitis,Antton Lamarca Arrizabalaga,Malte Thodberg,Maroulio Pertesi,Parashar Dhapola,Erik Bao,Abhishek Niroula,Divya Bali,Gudmundur Norddahl,Nerea Ugidos Damboriena,Vijay G Sankaran,Göran Karlsson,Unnur Thorsteinsdottir,Jonas Larsson,Kari Stefansson,Björn Nilsson

British journal of cancer 126:1205-1214 PubMed34837075

2021

MYB interacts with androgen receptor, sustains its ligand-independent activation and promotes castration resistance in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjeev Kumar Srivastava,Mohammad Aslam Khan,Shashi Anand,Haseeb Zubair,Sachin Kumar Deshmukh,Girijesh Kumar Patel,Seema Singh,Joel Andrews,Bin Wang,James Elliot Carter,Ajay Pratap Singh

Frontiers in oncology 11:761693 PubMed34868977

2021

The Clinical Significance and Transcription Regulation of a DNA Damage Repair Gene, SMC4, in Low-Grade Glioma Integrated Bioinformatic Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Wang,Zhisheng Wu

Cell death & disease 11:832 PubMed33028809

2020

LncRNA LL22NC03-N14H11.1 promoted hepatocellular carcinoma progression through activating MAPK pathway to induce mitochondrial fission.

Applications

Unspecified application

Species

Unspecified reactive species

Tingzhuang Yi,Hongcheng Luo,Fengxue Qin,Qi Jiang,Shougao He,Tonghua Wang,Jianwei Su,Sien Song,Xiaoshan Qin,Yueqiu Qin,Xihan Zhou,Zansong Huang

Blood cells, molecules & diseases 69:1-9 PubMed29227829

2017

A long noncoding RNA from the HBS1L-MYB intergenic region on chr6q23 regulates human fetal hemoglobin expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tasha A Morrison,Ibifiri Wilcox,Hong-Yuan Luo,John J Farrell,Ryo Kurita,Yukio Nakamura,George J Murphy,Shuaiying Cui,Martin H Steinberg,David H K Chui

Leukemia 32:882-889 PubMed29089643

2017

Targeting acute myeloid leukemia by drug-induced c-MYB degradation.

Applications

Unspecified application

Species

Unspecified reactive species

V Walf-Vorderwülbecke,K Pearce,T Brooks,M Hubank,M M van den Heuvel-Eibrink,C M Zwaan,S Adams,D Edwards,J Bartram,S Samarasinghe,P Ancliff,A Khwaja,N Goulden,G Williams,J de Boer,O Williams

Oncotarget 8:30138-30150 PubMed28404913

2017

A novel role of CKIP-1 in promoting megakaryocytic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Fan,Yiwu Wang,Yuan Shen,Qingyan Liu,Rong Gao,Ya Qiu,Chanjuan Wang,Lingqiang Zhang

Science signaling 7:ra47 PubMed24847116

2014

Targeting poly(ADP-ribose) polymerase and the c-Myb-regulated DNA damage response pathway in castration-resistant prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Likun Li,Wenjun Chang,Guang Yang,Chengzhen Ren,Sanghee Park,Theodoros Karantanos,Styliani Karanika,Jianxiang Wang,Jianhua Yin,Parantu K Shah,Hirayama Takahiro,Masato Dobashi,Wenling Zhang,Eleni Efstathiou,Sankar N Maity,Ana M Aparicio,Elsa M Li Ning Tapia,Patricia Troncoso,Bradley Broom,Lianchun Xiao,Hyun-Sung Lee,Ju-Seog Lee,Paul G Corn,Nora Navone,Timothy C Thompson

Oncogene 34:436-44 PubMed24469049

2014

TFAP2C governs the luminal epithelial phenotype in mammary development and carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

A R Cyr,M V Kulak,J M Park,M V Bogachek,P M Spanheimer,G W Woodfield,L S White-Baer,Y Q O'Malley,S L Sugg,A K Olivier,W Zhang,F E Domann,R J Weigel

Blood cells, molecules & diseases 52:68-75 PubMed23910958

2013

GFI1B, EVI5, MYB--additional genes that cooperate with the human BCL6 gene to promote the development of lymphomas.

Applications