Anti-c-Myb抗体(ab62824)
Key features and details
- Goat polyclonal to c-Myb
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Myb抗体
参阅全部 c-Myb 一抗 -
描述
山羊多克隆抗体to c-Myb -
宿主
Goat -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee, Zebrafish
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免疫原
Synthetic peptide:
C-QRHYNDEDPEKEKR
, corresponding to internal sequence amino acids 281-294 of Human c-Myb. -
阳性对照
- Recombinant Human c-Myb protein (ab114406) can be used as a positive control in WB. Human Spleen lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62824于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 3 - 6 µg/ml.
|
|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa).
1 hour primary incubation is recommended for this product. |
| 说明 |
|---|
|
IHC-P
Use a concentration of 3 - 6 µg/ml. |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 72 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 463016 Chimpanzee
- Entrez Gene: 317776 Cow
- Entrez Gene: 483995 Dog
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Entrez Gene: 498982 Rat
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Western blot - Anti-c-Myb antibody (ab62824)Anti-c-Myb antibody (ab62824) at 0.3 µg/ml + Human Spleen lysate in RIPA buffer at 35 µg
Predicted band size: 72 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Detected by chemiluminescence -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab62824)ab62824 at 4µg/ml staining c-Myb in paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
Inset image is a Negative Control showing paraffin embedded Human Kidney, with no primary antibody.
数据表及文件
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Datasheet download
文献 (4)
ab62824 被引用在 4 文献中.
- Srivastava SK et al. MYB interacts with androgen receptor, sustains its ligand-independent activation and promotes castration resistance in prostate cancer. Br J Cancer 126:1205-1214 (2022). PubMed: 34837075
- Wang F et al. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. Cell Res 27:416-439 (2017). PubMed: 28244490
- Yi T et al. eIF1A augments Ago2-mediated Dicer-independent miRNA biogenesis and RNA interference. Nat Commun 6:7194 (2015). WB . PubMed: 26018492
- Zheng J et al. Activation of hepatic stellate cells is suppressed by microRNA-150. Int J Mol Med 32:17-24 (2013). PubMed: 23604143
