Anti-c-Myb抗体(ab226470)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-c-Myb抗体
参阅全部 c-Myb 一抗 -
描述
兔多克隆抗体to c-Myb -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human c-Myb aa 1-50. The exact sequence is proprietary. (NP_005366.2).
Database link: P10242 -
阳性对照
- IP: MOLT-4 whole cell lysate. IHC-P: Human salivary gland and breast carcinoma tissues; Mouse renal cell carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab226470 was affinity purified using an epitope specific to c-Myb immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226470于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 463016 Chimpanzee
- Entrez Gene: 317776 Cow
- Entrez Gene: 483995 Dog
- Entrez Gene: 101136017 Gorilla
- Entrez Gene: 100073162 Horse
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Entrez Gene: 100452954 Orangutan
see all -
别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Immunoprecipitation - Anti-c-Myb antibody (ab226470)
c-Myb was immunoprecipitated from MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226470 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different rabbit anti-c-Myb antibody at 1 µg/ml.
Lane 1: ab226470 IP in MOLT-4 whole cell lysate.
Lane 2: Control IgG IP in MOLT-4 whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab226470)Formalin-fixed, paraffin-embedded mouse renal cell carcinoma tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab226470)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for c-Myb using ab226470 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab226470)Formalin-fixed, paraffin-embedded human salivary gland tissue stained for c-Myb using ab226470 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab226470 被引用在 3 文献中.
- Varadinkova S et al. Expression dynamics of metalloproteinases during mandibular bone formation: association with Myb transcription factor. Front Cell Dev Biol 11:1168866 (2023). PubMed: 37701782
- Uvarova AN et al. rs71327024 Associated with COVID-19 Hospitalization Reduces CXCR6 Promoter Activity in Human CD4+ T Cells via Disruption of c-Myb Binding. Int J Mol Sci 24:N/A (2023). PubMed: 37762093
- Kodric K et al. Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis. Exp Mol Med 51:1-16 (2019). PubMed: 31409771