Anti-c-Myb抗体(ab177510)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: IHC-P, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-c-Myb抗体
参阅全部 c-Myb 一抗 -
描述
兔多克隆抗体to c-Myb -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IPmore details -
种属反应性
与反应: Human
预测可用于: Cow, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide within Human c-Myb aa 175-225. The exact sequence is proprietary. (NP_005366.2)
Sequence:GRTDNA IKNHWNSTMR RKVEQEGYLQ ESSKASQPAV ATSFQKNSHL MGFAQ
Database link: P10242 -
阳性对照
- Human breast carcinoma tissue; MOLT4 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8. -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab177510 was affinity purified using an epitope specific to c-Myb immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab177510于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 317776 Cow
- Entrez Gene: 102137614 Cynomolgus monkey
- Entrez Gene: 101136017 Gorilla
- Entrez Gene: 4602 Human
- Entrez Gene: 100452954 Orangutan
- Entrez Gene: 712321 Rhesus monkey
- Omim: 189990 Human
- SwissProt: P46200 Cow
see all -
别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Immunoprecipitation - Anti-c-Myb antibody (ab177510)
Detection of Human c-Myb by Western Blot of Immunoprecipitate.
Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT4 cells.
Lane 2: Control IgG.
ab177510 used for IP at 6 μg/mg lysate.
ab177510 was used at 1 μg/ml for WB.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab177510)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue labeling c-Myb with ab177510 at 1µg/ml. Detection:DAB.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab177510 被引用在 1 文献中.
- Godfrey L et al. DOT1L inhibition reveals a distinct subset of enhancers dependent on H3K79 methylation. Nat Commun 10:2803 (2019). PubMed: 31243293