Anti-c-Myb抗体(ab117635)
Key features and details
- Rabbit polyclonal to c-Myb
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Myb抗体
参阅全部 c-Myb 一抗 -
描述
兔多克隆抗体to c-Myb -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse
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免疫原
KLH-conjugated synthetic peptide derived from the N terminal region of Human c-Myb.
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阳性对照
- Human breast or tonisl tissue. HUVEC cell lysate. HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 48% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117635于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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| IHC-P |
Use a concentration of 5 µg/ml.
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| ICC/IF | (1) |
1/100 - 1/500.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
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IHC-P
Use a concentration of 5 µg/ml. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells. -
序列相似性
Contains 3 HTH myb-type DNA-binding domains. -
结构域
Comprised of 3 domains; an N-terminal DNA-binding domain, a centrally located transcriptional activation domain and a C-terminal domain involved in transcriptional repression. -
翻译后修饰
Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
Phosphorylated by NLK on multiple sites, which induces proteasomal degradation. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 4602 Human
- Entrez Gene: 17863 Mouse
- Omim: 189990 Human
- SwissProt: P10242 Human
- SwissProt: P06876 Mouse
- Unigene: 606320 Human
- Unigene: 52109 Mouse
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别名
- C myb antibody
- c myb protein (140 AA) antibody
- c-myb protein (140 AA) antibody
see all
图片
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Western blot - Anti-c-Myb antibody (ab117635)All lanes : Anti-c-Myb antibody (ab117635) at 1/500 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HUVEC cell lysate with synthetic peptide
Predicted band size: 72 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-c-Myb antibody (ab117635)Immunofluorescence analysis of HeLa cells, using 1/100 ab117635.
The picture on the right is treated with the synthesized peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab117635)Human Breast, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myb antibody (ab117635)Human Tonsil, Formalin-Fixed, Paraffin-Embedded tissue, stained with ab117635 at 5µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab117635 被引用在 8 文献中.
- Eagle K et al. An oncogenic enhancer encodes selective selenium dependency in AML. Cell Stem Cell 29:386-399.e7 (2022). PubMed: 35108519
- Liu Q et al. Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter. J Cancer 12:1990-1999 (2021). PubMed: 33753997
- Simeoni F et al. Enhancer recruitment of transcription repressors RUNX1 and TLE3 by mis-expressed FOXC1 blocks differentiation in acute myeloid leukemia. Cell Rep 36:109725 (2021). PubMed: 34551306
- Kodric K et al. Sex-determining region Y (SRY) attributes to gender differences in RANKL expression and incidence of osteoporosis. Exp Mol Med 51:1-16 (2019). PubMed: 31409771
- Felipe-Abrio B et al. Loss of MYBBP1A Induces Cancer Stem Cell Activity in Renal Cancer. Cancers (Basel) 11:N/A (2019). PubMed: 30781655
- Xie R et al. c-Myb and its Effector COX-2 as an Indicator Associated with Prognosis and Therapeutic Outcome in Colorectal Cancer. J Cancer 10:1601-1610 (2019). PubMed: 31205515
- Ringleb J et al. Apoptotic Cancer Cells Suppress 5-Lipoxygenase in Tumor-Associated Macrophages. J Immunol 200:857-868 (2018). PubMed: 29229677
- Tosello V et al. A Novel t(8;14)(q24;q11) Rearranged Human Cell Line as a Model for Mechanistic and Drug Discovery Studies of NOTCH1-Independent Human T-Cell Leukemia. Cells 7:N/A (2018). PubMed: 30304769