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Anti-c-Kit (phospho Y703)抗体(ab62154)

Reviews (1)Q&A (7)References (8)
Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)
  • Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)

Key features and details

  • Rabbit polyclonal to c-Kit (phospho Y703)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-c-Kit (phospho Y703)抗体
    参阅全部 c-Kit 一抗

  • 描述

    兔多克隆抗体to c-Kit (phospho Y703)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse

  • 免疫原

    Synthetic peptide corresponding to Human c-Kit (phospho Y703). (A-L-YP-K-N)
    Database link: P10721

  • 阳性对照

    • WB: HepG2 treated with EGF cell lysate; Colon cancer lysate; U2OS lysate; Renal cancer cell line lysate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab62154于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Predicted molecular weight: 109 kDa.
说明
WB
1/500 - 1/1000. Predicted molecular weight: 109 kDa.

靶标

  • 功能

    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.

  • 组织特异性

    Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.

  • 疾病相关

    Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous

  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 翻译后修饰

    Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.

  • 细胞定位

    Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Information by UniProt

  • 数据库链接

    • 别名

      • C Kit antibody
      • c-Kit antibody
      • c-Kit Ligand antibody
      • CD117 antibody
      • Kit antibody
      • Kit Ligand antibody
      • KIT oncogene antibody
      • KIT proto oncogene receptor tyrosine kinase antibody
      • KIT_HUMAN antibody
      • Mast cell growth factor receptor antibody
      • Mast/stem cell growth factor receptor Kit antibody
      • MGF antibody
      • p145 c-kit antibody
      • PBT antibody
      • Piebald trait protein antibody
      • Proto oncogene c Kit antibody
      • Proto oncogene tyrosine protein kinase Kit antibody
      • Proto-oncogene c-Kit antibody
      • SCF Receptor antibody
      • SCFR antibody
      • soluble KIT variant 1 antibody
      • Steel Factor Receptor antibody
      • Stem cell factor receptor antibody
      • tyrosine protein kinase Kit antibody
      • Tyrosine-protein kinase Kit antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
      • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      see all

    图片

    • Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)
      Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)
      All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154) at 1/500 dilution

      Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) treated with EGF (200ng/ml, 30mins) cell lysate
      Lane 2 : HepG2 treated with EGF (200ng/ml, 30mins) cell lysate with immunizing phosphopeptide

      Predicted band size: 109 kDa

    • Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)
      Western blot - Anti-c-Kit (phospho Y703) antibody (ab62154)This image is courtesy of an Abreview submitted by Antibody Solutions
      All lanes : Anti-c-Kit (phospho Y703) antibody (ab62154)

      Lane 1 : Colon cancer lysate (human)
      Lane 2 : U2OS lysate (human)
      Lane 3 : Renal cancer cell line (human)


      Secondary
      All lanes : Swine anti-rabbit HRP at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 109 kDa


      Exposure time: 6 minutes

      See Abreview

    文献 (8)

    发表研究结果有使用 ab62154？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab62154 被引用在 8 文献中.

    • Shima T  et al. Glucose transporter‑1 inhibition overcomes imatinib resistance in gastrointestinal stromal tumor cells. Oncol Rep 47:N/A (2022). PubMed: 34738628
    • Zhao X  et al. Lactobacillus plantarum YS2 (yak yogurt Lactobacillus) exhibited an activity to attenuate activated carbon-induced constipation in male Kunming mice. J Dairy Sci 102:26-36 (2019). PubMed: 30527985
    • Yang H  et al. Predictive and prognostic value of phosphorylated c-KIT and PDGFRA in advanced non-small cell lung cancer harboring ALK fusion. Oncol Lett 17:3071-3076 (2019). PubMed: 30867736
    • Gong Y  et al. SCF/SCFR signaling plays an important role in the early morphogenesis and neurogenesis of human embryonic neural retina. Development 146:N/A (2019). PubMed: 31548215
    • Li G  et al. Preventative effects of fermented Chimonobambusa quadrangularis shoot on activated carbon-induced constipation. Exp Ther Med 13:1093-1100 (2017). WB . PubMed: 28450948
    • Yang S  et al. BMP4 promotes mouse iPS cell differentiation to male germ cells via Smad1/5, Gata4, Id1 and Id2. Reproduction 153:211-220 (2017). PubMed: 27864336
    • Grewal N  et al. Histopathology of aortic complications in bicuspid aortic valve versus Marfan syndrome: relevance for therapy? Heart Vessels 31:795-806 (2016). PubMed: 26129868
    • Grewal N  et al. Bicuspid aortic valve: phosphorylation of c-Kit and downstream targets are prognostic for future aortopathy. Eur J Cardiothorac Surg 46:831-9 (2014). PubMed: 25161185

    客户评价及客户问答

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    1-8 of 8 Abreviews or Q&A

    Western blot abreview for Anti-c-Kit (phospho Y703) antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Colon cancer (tissue lysate), U2OS (cell lysate) a)
    Loading amount
    20 µg
    Specification
    Colon cancer (tissue lysate), U2OS (cell lysate) a
    Gel Running Conditions
    Reduced Denaturing (Pierce precise protein gel 4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mr. Antibody Solutions

    Verified customer

    提交于 Apr 20 2011

    Question

    Thank you so much for your help. I really appreciate your understanding.  I will have our purchase department contact your account team.

    Read More
    Answer

    It is my pleasure to help.

    Please let me know if you have any further questions or if there are any ways in which Abcam might help you in the future.

    Read More

    Question

    Hi, Thank you for your note for give us the credit.  However, this is the large amount of money.  We are not going to purchase anything that much from Abcam anymore.  Could you just refund the money back to my account please?  In fact, we might have to close the lab in the next few month, unless our RO1 application get approve.  Refund would be the best for us, not credit so we can use for immediately to pay for staining service at IHC core. Thank you,

    Read More
    Answer

    This will not be a problem. Our accounts team can gladly arrange thisfor you. Please have your purchase department contact our accounts team at the email address which was included in the credit note details or by telephone at 888-772-2226.



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

    Question

      Below is my respond to your questions.   As you had ordered a single vial in July followed by 4 more in August, am I correct in assuming that the first vial did give good specific result but subsequent ones did not? Did you test each the vials from you August order to determine whether this was vial specific problem? I ask as these facts are important for us to determine if there was something which may have happened to the subsequent vials which might have affected them. The first vial was tested on breast carcinoma tissue.  There was some non specific staining in nucleus of cancer tissue as well as normal tissue.  However, we decided to go ahead with our sample (Adenoid cystic carcinoma of Salivary gland) WITH the isotype control staining.  The isotype control should help us identify the non-specific staining and we can read the signal from specific antibody.  We ordered one vial of isotype control and 4 vials of p-c-kit antibody, the same lot number with the first one.  We pooled the entire antibody and stain all 35 slides.  The isotype control was used in the same concentration and uses the same protocol for 6 slides.   The test sample and isotype control were positive in cancer tissue, normal tissue, nucleus, cytoplasm and cell membrane for all slides with stronger signals than the test samples.  The pathologist was not able to interpret the result from this staining.    Thank you for providing information regarding the dilution, tissue, control and detection system which you have used with this product. If you don't mind, would you be able to tell me the antigen retrieval, blocking, antibody incubation and washing conditions which you have used as well. These details will help us should we need to have the product re-submitted for quality control. I am attached the protocol that UCSF Cancer Center IHC core use for my staining with this mail.   I would also like to remind you that if any Abcam product does not work for you, please contact us immediately. Our products are covered by our six month Abpromise in which we not only provide support but are able to replace or refund in that period if they are being used in applications and species listed on the datasheet.  We ordered one vial in 7/23/12.  We tested on breast carcinoma tissue with 1:100, 1:50 and 1:25 on 8/2/12.  The pathologist saw only 1+ signal from the sample.  She asked us to perform again with higher concentration to increase the signal.  We performed the IHC again with 1:20, 1:10 and 1:5 on 8/8/12.  This time, she saw the 2+to 3+ signals in 1:10 dilution. I ordered the antibody again, along with isotype control on 8/13/12.  We received the antibody on 8/21/12.  The slides were stained on 8/23/12 and reviewed by pathologist on 8/27/12.  I reported the problem to you the same day-yesterday.     We do understand the financial pressures felt by researchers these days which is why we are happy to have Abpromise in place. While we are able to refund Abcam products, under our terms and conditions we cannot compensate for additional costs. " Any claim relating to products shall be limited to replacement or refund of original purchase price paid. " I do look forward to hearing from you these additional protocol details. If you could please confirm the Abcam order confirmation numbers or POs, this would be of great help in moving forward as well. The first order PO is B000094576 received on 7/23/12. The second order PO is B000104345 received on 8/21/12.   Thank you in advance for your help. Please let me know if you have any other questions.  

    Read More
    Answer

    Thank you for contacting us. Your credit note ID are xxxx and xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Question

    I have a complain about your product phospho c-kit antibody (ab62154).  I have been working with this antibody, using the different dilution (1:5 to 1:100) and different breast carcinoma tissue as well as different DAP developing time.  We have the best dilution and last week, along with isotype control staining, we completed the staining for my samples.  Unfortunately, we cannot use any of our data from the staining due to several reasons.   1.  The antibody are stain non-specific.  The positive signal should be only on the cell membrane, however they are stain all in nucleus, cytoplasm and cell membrane.  The pathologist is not able to evaluate the significant of this staining. 2.  The isotype staining is very strong.  The signal from isotype staining is much more than the specific antibody.  This make it impossible for interpret any signal from specific antibody.   I have invested a lot of my time, money and energy for this staining.  I spent the money for 6 tubes of antibodies, over $1200 for IHC core to stain the sample for optimization and test, $300 for section preparation and most of all, my FFPE tissue sample which is the rare cases.  We have only 33 cases for the last 20 years archive.    I am writing this e-mail to ask for the reimbursement of the money that I spent for at least the antibody part from Abcam.  Would you please consider refund the money for me?  As you know, all the lab has very hard time with money issue now.  Spending more than $6000 and time is not acceptable for me.  I can send in the slide if you would like to, there is no use in the lab.

    Read More
    Answer

    My name is Keith. John is away for a few weeks and as such I will be helping you with this problem.

    First, I want to express my sympathy to you. I do understand the frustration which comes from this type of problem and want to help resolve this issue.

    As you had ordered a single vial in July followed by 4 more in August, am I correct in assuming that the first vial did give good specific result but subsequent ones did not? Did you test each the vials from you August order to determine whether this was vial specific problem? I ask as these facts are important for us to determine if there was something which may have happened to the subsequent vials which might have affected them.

    Thank you for providing information regarding the dilution, tissue, control and detection system which you have used with this product. If you don't mind, would you be able to tell me the antigen retrieval, blocking, antibody incubation and washing conditions which you have used as well. These details will help us should we need to have the product re-submitted for quality control.

    I would also like to remind you that if any Abcam product does not work for you, please contact us immediately. Our products are covered by our six month Abpromise in which we not only provide support but are able to replace or refund in that period if they are being used in applications and species listed on the datasheet.

    We do understand the financial pressures felt by researchers these days which is why we are happy to have Abpromise in place. While we are able to refund Abcam products, under our terms and conditions we cannot compensate for additional costs. "Any claim relating to products shall be limited to replacement or refund of original purchase price paid." (https://www.abcam.com/index.html?pageconfig=terms)

    I do look forward to hearing from you these additional protocol details. If you could please confirm the Abcam order confirmation numbers or POs, this would be of great help in moving forward as well. Please let me know if you have any

    Read More

    Question

    I am working with p-c-kit (ab62154). I will use this antibody in 1:10 dilution (100 ug/ml) to stain my 40 slides sample. In the same time, I am looking for the isotype control of this antibody which I should use the same concentration. However, your isotype control below is only 2ug/ml which far too low to use.



    Do you have any suggestion for me here? Can you concentrate the rabbit IgG for me or have other product that is higher than 100 ug/ml available.

    Read More
    Answer

    Thank you for your reply.

    We do have a few other rabbit polyclonal isotype controls that are at a higher concentration, these are:

    https://www.abcam.com/Rabbit-polyclonal-IgG-ab27478.html
    https://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html



    If there is anything else I can help you with, or if there are any other products I can help you select, please let me know.

    Read More

    Question

    I have another question regarding this antibody. I need to have the isotype control of this antibody. Do you know what the subtype of this antibody is? In datasheet, it indicate only rabbit IgG. I am hoping to know more detail of IgG so I can have the subtype control. I also want to know the catalog number of this control as well.

    Read More
    Answer

    Thank you for your reply.

    As this is a rabbit polyclonal antibody, the exact clonality of the antibody cannot be determined. However we do have isotype controls that would work for you, please see the link below:


    https://www.abcam.com/Rabbit-polyclonal-IgG-ab27472.html



    If there is anything else I can help you with, please let me know.

    Read More

    Question

    I am interested in using anti-c-kit (phosphor Y703) antibody (ab62154) for IHC with our paraffin-embedded tissue. I have a few questions regarding this antibody.



    1. In the datasheet, you state that you use the human breast carcinoma tissue as the positive control. Can I have more information of what kind of breast carcinoma tissue are you using? I can have the same or close one as the positive control for my staining as well.



    2. I also would like to know if you have any reference of the papers that use this antibody for their staining. If so, I would like to have that paper(s) for my reference.



    3. Do you have the protocol for this antibody? I have very precious rare cancer samples. I am planning to do the optimize staining with breast cancer first before use with my sample, but having the protocol would be very helpful.

    Read More
    Answer

    Thank you for contacting Abcam.

    I have attached the IHC-P protocol that was used to test ab62154 and currently we do not know if any publications that have used this antibody in IHC-P, that does not mean there are not any, just that we do not know.

    Also, I do not have any further information about the breast cancer samples that was used.

    I am sorry that I could not help further in this matter, but please let me know if you have any further questions.

    Read More

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