Anti-c-Jun抗体(ab31419)
Key features and details
- Rabbit polyclonal to c-Jun
- Suitable for: ELISA, ICC/IF, IP, ChIP, Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Jun抗体
参阅全部 c-Jun 一抗 -
描述
兔多克隆抗体to c-Jun -
宿主
Rabbit -
特异性
Antibody detects endogenous levels of c-Jun protein around Serine 243. -
经测试应用
适用于: ELISA, ICC/IF, IP, ChIP, Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human, African green monkey
预测可用于: Chicken, Cow, Pig
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免疫原
Synthetic peptide within Human c-Jun aa 210-259. The exact sequence is proprietary.
Sequence:HLPQQMPVQHPRLQALKEEPQTVPEMPGETPPLSPIDMESQERIKAERKR
Database link: P05412 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Corresponding phospho antibody
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31419于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| IP | (2) |
Use at an assay dependent concentration.
|
| ChIP | (1) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| IHC-P | (2) |
1/50 - 1/100.
|
| WB | (7) |
1/500 - 1/1000. Predicted molecular weight: 36 kDa.
|
| 说明 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 1 µg/ml. |
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IP
Use at an assay dependent concentration. |
|
ChIP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/100. |
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WB
1/500 - 1/1000. Predicted molecular weight: 36 kDa. |
靶标
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功能
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
序列相似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻译后修饰
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 424673 Chicken
- Entrez Gene: 280831 Cow
- Entrez Gene: 3725 Human
- Entrez Gene: 16476 Mouse
- Entrez Gene: 396913 Pig
- Entrez Gene: 24516 Rat
- Omim: 165160 Human
- SwissProt: P18870 Chicken
see all -
别名
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (ab31419)
Paraffin-embedded human breast carcinoma tissue stained for c-Jun with ab31419 at a 1/50 dilution in immunohistochemical analysis.
Left panel: Untreated.
Right panel: Pre-incubated with synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody (ab31419)ICC/IF image of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling c-Jun (green) with ab31419 at 1 µg/ml. The cells were fixed in 4% PFA (10 minutes) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with ab31419 at 1 µg/ml overnight at +4 °C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) ab150077 used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
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Western blot - Anti-c-Jun antibody (ab31419)All lanes : Anti-c-Jun antibody (ab31419) at 1/500 dilution
Lane 1 : Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells
Lane 2 : Extracts of Hela (Human epithelial cell line from cervix adenocarcinoma) cells with immunizing peptide
Predicted band size: 36 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted? -
Western blot - Anti-c-Jun antibody (ab31419)Anti-c-Jun antibody (ab31419) at 1/500 dilution + Recombinant Human c-Jun protein (ab54318) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Exposure time: 30 seconds
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (89)
ab31419 被引用在 89 文献中.
- Li D et al. c-Jun as a one-way valve at the naive to primed interface. Cell Biosci 13:191 (2023). PubMed: 37838693
- Fisher AL et al. Quantitative proteomics and RNA-sequencing of mouse liver endothelial cells identify novel regulators of BMP6 by iron. iScience 26:108555 (2023). PubMed: 38125029
- Qi Z et al. Protection of toll-like receptor 9 against lipopolysaccharide-induced inflammation and oxidative stress of pulmonary epithelial cells via MyD88-mediated pathways. Physiol Res 71:259-273 (2022). PubMed: 35275698
- Liu Z et al. Epigenetic Regulation of NGF-Mediated Osteogenic Differentiation in Human Dental Mesenchymal Stem Cells. Stem Cells 40:818-830 (2022). PubMed: 35728620
- Javellana M et al. Neoadjuvant Chemotherapy Induces Genomic and Transcriptomic Changes in Ovarian Cancer. Cancer Res 82:169-176 (2022). PubMed: 34737212