Anti-c-Jun抗体(ab31367)
Key features and details
- Rabbit polyclonal to c-Jun
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Jun抗体
参阅全部 c-Jun 一抗 -
描述
兔多克隆抗体to c-Jun -
宿主
Rabbit -
特异性
ab31367 detects endogenous levels of c-Jun protein around Serine 73. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human c-Jun. Range of aa 40-89 Synthetic non-phosphopeptide derived from human c-Jun around the phosphorylation site of Serine 73.
Database link: P05412 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Corresponding phospho antibody
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab31367于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
|
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| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
序列相似性
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
翻译后修饰
Ubiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3725 Human
- Omim: 165160 Human
- SwissProt: P05412 Human
- Unigene: 696684 Human
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别名
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody (ab31367)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma labeling c-Jun with ab31367 at 1/100. Left: c-Jun antibody, Right: Preincubated with synthesized peptide.
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Western blot - Anti-c-Jun antibody (ab31367)All lanes : Anti-c-Jun antibody (ab31367) at 1/1000 dilution
Lane 1 : HeLa cells lysate, preincubated with immunogen (synthetic non-phosphopeptide)
Lane 2 : HeLa cells lysate
Predicted band size: 36 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab31367 被引用在 4 文献中.
- Ting W et al. Overexpression of microRNA-203 Suppresses Proliferation, Invasion, and Migration while Accelerating Apoptosis of CSCC Cell Line SCL-1. Mol Ther Nucleic Acids 21:428-440 (2020). PubMed: 32668390
- Yuan D et al. Kupffer Cell-Derived Tnf Triggers Cholangiocellular Tumorigenesis through JNK due to Chronic Mitochondrial Dysfunction and ROS. Cancer Cell 31:771-789.e6 (2017). PubMed: 28609656
- Winter C et al. Google goes cancer: improving outcome prediction for cancer patients by network-based ranking of marker genes. PLoS Comput Biol 8:e1002511 (2012). IHC-Fr ; Human . PubMed: 22615549
- Samson WK et al. Neuronostatin encoded by the somatostatin gene regulates neuronal, cardiovascular, and metabolic functions. J Biol Chem 283:31949-59 (2008). IHC-P ; Mouse . PubMed: 18753129