重组Anti-c-Fos抗体[EPR883(2)] (ab134122)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR883(2)] to c-Fos
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-c-Fos抗体[EPR883(2)]
参阅全部 c-Fos 一抗 -
描述
兔单克隆抗体[EPR883(2)] to c-Fos -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human c-Fos aa 200-300. The exact sequence is proprietary.
-
阳性对照
- Lysates of U937 cells treated with TPA and untreated U937 cells, Lysate of HeLa cells treated with TPA.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR883(2) -
同种型
IgG -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134122于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/1000 - 1/10000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 41 kDa).
|
说明 |
---|
Flow Cyt (Intra)
1/1000 - 1/10000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular weight: 41 kDa). |
靶标
-
功能
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. -
序列相似性
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain. -
翻译后修饰
Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation.
Constitutively sumoylated by SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 2353 Human
- Entrez Gene: 14281 Mouse
- Entrez Gene: 314322 Rat
- Omim: 164810 Human
- SwissProt: P01100 Human
- SwissProt: P01101 Mouse
- SwissProt: P12841 Rat
- Unigene: 246513 Mouse
see all -
别名
- Activator protein 1 antibody
- AP 1 antibody
- C FOS antibody
see all
图片
-
All lanes : Anti-c-Fos antibody [EPR883(2)] (ab134122) at 1/1000 dilution
Lane 1 : Wild-type PC-12 Untreated Control NGF (0 ng/mL, 4 days) cell lysate
Lane 2 : Wild-type PC-12 Treated NGF (111 ng/mL, 4 days) cell lysate
Lane 3 : FOS knockout PC-12 Untreated Control NGF (0 ng/mL, 4 days) cell lysate
Lane 4 : FOS knockout PC-12 Treated NGF (111 ng/mL, 4 days) cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 45-55 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-c-Fos antibody [EPR883(2)] staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab134122 was shown to bind specifically to c-Fos. A band was observed at 45-55 kDa in treated wild-type PC-12 cell lysates with no signal observed at this size in FOS knockout cell line ab281615 (knockout cell lysate ab283111). Treatment of NGF has no observable affect on protein expression in this cell line. To generate this image, wild-type and FOS knockout PC-12 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
-
All lanes : Anti-c-Fos antibody [EPR883(2)] (ab134122) at 1/1000 dilution
Lane 1 : Lysate of U937 cells treated with TPA
Lane 2 : U937 cell lysate
Lane 3 : Lysate of HeLa cells treated with TPA
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat ant-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Overlay histogram showing HeLa cells stained with ab134122 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134122, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
数据表及文件
-
SDS download
-
Datasheet download
文献 (37)
ab134122 被引用在 37 文献中.
- Oelschläger L et al. Taspase1 Facilitates Topoisomerase IIβ-Mediated DNA Double-Strand Breaks Driving Estrogen-Induced Transcription. Cells 12:N/A (2023). PubMed: 36766705
- Shou X et al. miR-126 promotes M1 to M2 macrophage phenotype switching via VEGFA and KLF4. PeerJ 11:e15180 (2023). PubMed: 37020848
- Yang F et al. Casticin suppresses RANKL‑induced osteoclastogenesis and prevents ovariectomy‑induced bone loss by regulating the AKT/ERK and NF‑κB signaling pathways. Int J Mol Med 51:N/A (2023). PubMed: 37052260
- Xu M et al. CGK733 alleviates ovariectomy-induced bone loss through blocking RANKL-mediated Ca2+ oscillations and NF-κB/MAPK signaling pathways. iScience 26:107760 (2023). PubMed: 37720109
- Yang T et al. Myrislignan targets extracellular signal-regulated kinase (ERK) and modulates mitochondrial function to dampen osteoclastogenesis and ovariectomy-induced osteoporosis. J Transl Med 21:839 (2023). PubMed: 37993937