Anti-c-Fos 抗体 - BSA free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody - BSA free (AB190289)

Immunocytochemistry analysis of HeLa cells labeling c-Fos with ab190289 at 1/5000 dilution (red) and mouse mAb to tubulin at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) – free media for 36 hours. Then the cells were treated with PBS (A) as a control or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody - BSA free (AB190289)

Immunofluorescent analysis of HeLa cells (serum–starved 36 hours followed by 20% FBS treatment for 2 hours) labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with chicken polyclonal antibody against vimentin (green) and DAPI (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody - BSA free (AB190289)

Immunohistochemistry analysis of mouse brain sections across hippocampus labeling c-Fos with ab190289 at 1/2000 dilution (red). Cells were counterstained with anti Fox3/NeuN antibody (green) and DAPI (blue). Neurons positive for c-Fos and Fox3/NeuN appear to be yellow. Inset shows an enlarged image of c-Fos staining.

• IHC-FrFl

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Immunohistochemistry - Free Floating - Anti-c-Fos antibody - BSA free (AB190289)

Immunohistochemistry - Free Floating analysis of mouse hippocampus (C) or olfactory bulb sections (D) labeling c-Fos wiht ab190289 at 1/20,000 (red) and mouse mAb to NF-L at 1/5,000 (green). The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45μM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.

• WB

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Western blot - Anti-c-Fos antibody - BSA free (AB190289)

All lanes:

Western blot - Anti-c-Fos antibody - BSA free (ab190289) at 1/2000 dilution

Lane 1:

HeLa cell lysate from cells serum-starved for 36 hours

Lane 2:

HeLa cell lysate from cells serum-starved for 36 hours then stimulated with 20% fetal bovine serum for 2 hours

Predicted band size: 41 kDa

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• WB

Unknown

Western blot - Anti-c-Fos antibody - BSA free (AB190289)

Mouse Monoclonal Antibody to GAPDH, dilution 1/5000, was used as a loading control (red).

Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein.

All lanes:

Western blot - Anti-c-Fos antibody - BSA free (ab190289) at 1/2000 dilution

Lane 1:

Protein standard

Lane 2:

HeLa cells grown in FBS free media

Lane 3:

HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours

Lane 4:

Rat cortical neurons

Lane 5:

Rat cortical neurons treated with membrane depolarization buffer for 5 hours

Predicted band size: 41 kDa

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• WB

CiteAb

Western blot - Anti-c-Fos antibody - BSA free (AB190289)

Western Blotting using Anti-c-Fos antibody - BSA free, ab190289. Publication image from Sun, X. et al., 2020, Front Pharmacol, 32292342. Legend direct from paper.

Pregnenolone (Preg) inhibited the RANKL-induced activation of ERK and NF-κB signaling cascades, and the downstream induction of c-Fos and NFATc1. (A) To examine early RANKL signaling events, BMMs were serum-starved for 1 h, pre-treated with 20 μM Preg for 3 h, and then stimulated with RANKL for the indicated time. Total cellular protein were extracted and subjected to western blot analyses using specific antibodies against ERK and p-ERK, p38 and p-p38, JNK and p-JNK, IκBα, NF-κB p65 and p-p65, and β-actin. (B–F) Quantitative densitometric analysis of phosphorylated protein to total protein counterpart, or normalized to β-actin were conducted. (G) To examine downstream (late stage) RANLK signaling events, BMMs were treated with RANKL for 0, 1, 3, and 5 d. M-CSF-dependent BMMs were stimulated with RANKL without or with 10 and 20 μM Preg for 72 h. Total cellular proteins were extracted and subjected to western blot analyses using specific antibodies against NFATc1, c-Fos, and GAPDH. (H, I) Quantitative densitometric analysis of (h) NFATc1 and (i) c-Fos normalized to GAPDH were conducted. Values presented as the mean ± standard deviation (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001.

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