Anti-Butyrylcholinesterase抗体[OTI4C12] (ab117960)
![Western blot - Anti-Butyrylcholinesterase antibody [OTI4C12] (ab117960)](https://www.abcam.cn/ps/products/117/ab117960/Images/ab117960-129741-anti-butyrylcholinesterase-antibody-oti4c12-western-blot.jpg "Western blot - Anti-Butyrylcholinesterase antibody [OTI4C12] (ab117960)")
Key features and details
- Mouse monoclonal [OTI4C12] to Butyrylcholinesterase
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Butyrylcholinesterase抗体[OTI4C12]
参阅全部 Butyrylcholinesterase 一抗 -
描述
小鼠单克隆抗体[OTI4C12] to Butyrylcholinesterase -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Butyrylcholinesterase. Produced in HEK293T cells (NP_000046).
Database link: P06276 -
阳性对照
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY butyrylcholinesterase cDNA.
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常规说明
Clone OTI4C12 (formerly 4C12).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
纯度
Affinity purified -
纯化说明
Purified from cell culture supernatant by affinity chromatography. -
克隆
单克隆 -
克隆编号
OTI4C12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117960于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 68 kDa.
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| 说明 |
|---|
|
WB
1/2000. Predicted molecular weight: 68 kDa. |
靶标
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功能
Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. -
组织特异性
Detected in blood plasma (at protein level). Present in most cells except erythrocytes. -
疾病相关
Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency) [MIM:177400]. BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 590 Human
- Omim: 177400 Human
- SwissProt: P06276 Human
- Unigene: 420483 Human
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别名
- Acylcholine acylhydrolase antibody
- BCHE antibody
- Butyrylcholine esterase antibody
see all
图片
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Western blot - Anti-Butyrylcholinesterase antibody [OTI4C12] (ab117960)All lanes : Anti-Butyrylcholinesterase antibody [OTI4C12] (ab117960) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY Butyrylcholinesterase cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 68 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab117960 尚未被引用在任何文献中。