重组Anti-BubR1 (phospho S670)抗体[EPR20109] - BSA and Azide free (ab223150)
![Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)](https://www.abcam.cn/ps/products/223/ab223150/Images/ab223150-320813-anti-bubr1-phospho-s670-antibody-epr20109-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20109] to BubR1 (phospho S670) - BSA and Azide free
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BubR1 (phospho S670)抗体[EPR20109] - BSA and Azide free
参阅全部 BubR1 一抗 -
描述
兔单克隆抗体[EPR20109] to BubR1 (phospho S670) - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa whole cell lysate treated with 0.5 µM nocodazole for 24 hours. ICC/IF: HeLa cells.
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常规说明
ab223150 is the carrier-free version of ab200062.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR20109 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223150于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa).
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| ICC/IF |
1/250.
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| 说明 |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). |
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ICC/IF
1/250. |
靶标
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功能
Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. -
组织特异性
Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. -
疾病相关
Note=Defects in BUB1B are associated with tumor formation.
Defects in BUB1B are the cause of premature chromatid separation trait (PCS) [MIM:176430]. PCS consists of separate and splayed chromatids with discernible centromeres and involves all or most chromosomes of a metaphase. It is found in up to 2% of metaphases in cultured lymphocytes from approximately 40% of normal individuals. When PCS is present in 5% or more of cells, it is known as the heterozygous PCS trait and has no obvious phenotypic effect, although some have reported decreased fertility. Inheritance is autosomal dominant.
Defects in BUB1B are the cause of mosaic variegated aneuploidy syndrome (MVA) [MIM:257300]. MVA is a severe autosomal recessive developmental disorder characterized by mosaic aneuploidies, predominantly trisomies and monosomies, involving multiple different chromosomes and tissues. The proportion of aneuploid cells varies but is usually more than 25% and is substantially greater than in normal individuals. Affected individuals typically present with severe intrauterine growth retardation and microcephaly. Eye anomalies, mild dysmorphism, variable developmental delay, and a broad spectrum of additional congenital abnormalities and medical conditions may also occur. The risk of malignancy is high, with rhabdomyosarcoma, Wilms tumor and leukemia reported in several cases. MVA is caused by biallelic mutations in the BUB1B gene. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.
Contains 1 BUB1 N-terminal domain.
Contains 1 protein kinase domain. -
结构域
The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.
The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3. -
翻译后修饰
Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.
Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.
Ubiquitinated. Degradated by the proteasome.
Sumoylated by SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.
Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase. -
细胞定位
Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and CASC5. - Information by UniProt
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数据库链接
- Entrez Gene: 701 Human
- Omim: 602860 Human
- SwissProt: O60566 Human
- Unigene: 513645 Human
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别名
- Beta homolg of S. cerevisiae BUB 1 antibody
- Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles antibody
- BUB 1B antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho S670) with ab200062 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing strong signal on mitotic phase of HeLa cells, the expression decreased after treatment with lambda protein phosphatase 31°C for 2h.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200062).
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![Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)](https://www.abcam.cn/ps/products/223/ab223150/Images/ab223150-320812-anti-bubr1-phospho-s670-antibody-epr20109-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BubR1 (phospho S670) with ab200062 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing strong signal on mitotic phase of HeLa cells, the expression decreased after treatment with lambda protein phosphatase 31°C for 2h.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200062).
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![Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)](https://www.abcam.cn/ps/products/223/ab223150/Images/ab223150-3-benefits-of-recombinant-antibodies.png)
Anti-BubR1 (phospho S670) antibody [EPR20109] - BSA and Azide free (ab223150)
实验方案
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab223150 尚未被引用在任何文献中。
